The Effect of Omega-3 Supplementation on Muscle Functioning, Inflammation and Muscle Signaling

يمكن للمستخدمين المسجلين فقط ترجمة المقالات

الدخول التسجيل فى الموقع

يتم حفظ الارتباط في الحافظة

الحالةمنجز

الرعاة

KU Leuven

تجربة سريرية: NCT03986307

BioSeek: NCT03986307

الكلمات الدالة

نبذة مختصرة

Elderly are supplemented with either Omega-3 (3 x 1.1g per day) or placebo (corn oil) during 14 weeks. The last 12 weeks, supplementation is combined with resistance exercise training (3x per week) focusing on upper leg strength. In the present project, the investigators study whether differences in muscle strength and/or muscle mass between conditions can be explained by omega-3-induced effects on systemic or muscle inflammatory signaling, or differences in muscle molecular signaling.

وصف

تواريخ

آخر التحقق:	05/31/2019
تم الإرسال لأول مرة:	06/03/2019
تم إرسال التسجيل المقدر:	06/11/2019
أول نشر:	06/13/2019
تم إرسال آخر تحديث:	06/11/2019
آخر تحديث تم نشره:	06/13/2019
تاريخ بدء الدراسة الفعلي:	12/31/2018
تاريخ الإنجاز الأساسي المقدر:	05/24/2019
التاريخ المتوقع لانتهاء الدراسة:	05/24/2019

حالة أو مرض

Aging

التدخل / العلاج

Dietary Supplement: Omega-3

Other: Resistance exercise

Dietary Supplement: Placebo

مرحلة

مجموعات الذراع

ذراع	التدخل / العلاج
Experimental: Omega-3 Elderly supplemented with omega-3.	Dietary Supplement: Omega-3 Daily supplementation with omega-3 capsules (daily for 14 weeks). Each day: 3 capsules of 1.1g
Placebo Comparator: Placebo Elderly supplemented with corn oil.	Dietary Supplement: Placebo Daily supplementation with placebo capsules (daily for 14 weeks). Each day: 3 capsules of 1.1g

معايير الأهلية

الأعمار المؤهلة للدراسة	65 Years إلى 65 Years
الأجناس المؤهلة للدراسة	All
يقبل المتطوعين الأصحاء	نعم
المعايير	Inclusion Criteria: - aged >= 65 years Exclusion Criteria: - BMI < 20; BMI > 35 - regular NSAID intake in the last 3 months - omega-3 supplementation in the last year - regular resistance exercise in the last year - cancer, liver and renal disease, unstable cardiovascular disease, musculoskeletal disease - cognitive limitations that might interfere with the (adherence to) the intervention or with the testing of outcome parameters - unstable body weight - smoking

النتيجة

مقاييس النتائج الأولية

1. Isometric muscle strength [Change in muscle strength between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Isometric muscle strength as assessed with Biodex Device in Nm

2. Systemic inflammatory signaling [Change in inflammatory signaling between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Systemic (blood) markers of inflammation: hs-CRP (mg/L), IL-6 (pg/mL), TNFa (pg/mL)

3. Muscle strength [Change in muscle strength between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

One Repetition Maximum on the leg press fitness device in kg

مقاييس النتائج الثانوية

1. Muscle quality [Change in muscle quality between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

muscle strength (Torque) per muscle volume

2. Muscle molecular signaling (Protein levels of anabolic/catabolic signaling and skeletal muscle stress) [Change in muscle molecular signaling between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Biochemical analyses of muscle biopsies obtained pre-post intervention in both conditions. This involves parameters of muscle anabolism, catabolism, stress, structure, integrity, quality etc.: mTORC1 pathway, FoXO, MAPK (western blotting technique, values expressed as arbitrary units)

تدابير النتائج الأخرى

1. Handgrip strength [Change in handgrip strength between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Handgrip strength measured by a dynamometer (results in kg)

2. Timed up-and-go test [Change in timed up-and-go test performance between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Timed-up-and-go: time needed to stand up from a chair, walk 3m, turn, walk back and sit down.

3. Sit-to-stand test [Change in sit-to-stand test performance between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Sit-to-stand: the amount of repetitions that one can complete during 30 sec and the time needed to complete 5 sit-to-stands.

4. Fat% [Change in fat% between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Bioelectrical impedance analysis-derived fat %

5. Lean mass% [Change in lean mass% between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Bioelectrical impedance analysis-derived lean mass %

6. Basal metabolic rate [Change in basal metabolic rate between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Bioelectrical impedance analysis-basal metabolic rate (kcal)

7. Muscle Volume Left [Change in muscle volume between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Muscle Volume in a 2-cm CT-scan slice (4 slices of 5mm)

8. Mean Muscle Hounsfield Unit [Change in Mean Muscle Hounsfield Unit between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Muscle Hounsfield Unit serves as a marker of muscle quality

9. Sleep [Change in sleep quality between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Questionnaire (Pittsburgh Sleep Quality Index)

10. Stress [Change inperceived stress between prior to and following the study intervention of 14 weeks (Jan - May 2019)]

Questionnaire (Perceived Stress Scale)