Azerbaijani
Albanian
Arabic
Armenian
Azerbaijani
Belarusian
Bengali
Bosnian
Catalan
Czech
Danish
Deutsch
Dutch
English
Estonian
Finnish
Français
Greek
Haitian Creole
Hebrew
Hindi
Hungarian
Icelandic
Indonesian
Irish
Italian
Japanese
Korean
Latvian
Lithuanian
Macedonian
Mongolian
Norwegian
Persian
Polish
Portuguese
Romanian
Russian
Serbian
Slovak
Slovenian
Spanish
Swahili
Swedish
Turkish
Ukrainian
Vietnamese
Български
中文(简体)
中文(繁體)

Choline Dehydrogenase and Sperm Function: Effects of Betaine

Yalnız qeydiyyatdan keçmiş istifadəçilər məqalələri tərcümə edə bilərlər
Giriş / Qeydiyyatdan keçin
Bağlantı panoya saxlanılır
StatusTamamlandı
Sponsorlar
University of North Carolina, Chapel Hill

Açar sözlər

Mücərrəd

The ability of sperm to swim is important for normal fertility. Men with a genetic variation in the gene coding for Choline Dehydrogenase (CHDH) have decreased energy production by sperm, and their sperm do not swim normally. The metabolic product of this gene is a nutrient called betaine (found normally in the diet as a part of many foods such as spinach, beets and grain products). This study tests whether treatment with betaine is safe and whether it can normalize energy production in sperm of these men and restore normal swimming ability.

Təsvir

Unidentified genetic aberrations such as single nucleotide polymorphisms (SNPs) may be the underlying cause of many cases of idiopathic infertility in men. Choline dehydrogenase (encoded by CHDH) converts choline to betaine in the mitochondria. 5-9% of men have 2 alleles for a functional SNP in CHDH (rs12676), and they have low sperm adenosine triphosphate (ATP) concentrations with impaired sperm motility (asthenospermia) that should decrease fertility. Male mice in which CHDH is deleted also have very low sperm ATP, asthenospermia and are infertile. Supplementation of these mice with dietary betaine increases sperm motility and ATP concentrations.

This purpose of this study is to conduct a phase I study of betaine treatment in men with 2 minor alleles for CHDH rs12676 to determine whether betaine supplementation is safe and to obtain preliminary data on the effects of betaine on sperm mitochondrial ATP concentrations and sperm motility in these men.

Tarixlər

Son Doğrulandı: 08/31/2016
İlk təqdim: 04/16/2014
Təxmini qeydiyyat təqdim edildi: 04/21/2014
İlk Göndərmə: 04/23/2014
Son Yeniləmə Göndərildi: 09/06/2016
Son Yeniləmə Göndərildi: 09/07/2016
Həqiqi Təhsilin Başlama Tarixi: 03/31/2014
Təxmini İlkin Tamamlanma Tarixi: 06/30/2016
Təxmini İşin Tamamlanma Tarixi: 06/30/2016

Vəziyyət və ya xəstəlik

Men Carrying 2 Minor Alleles for Choline Dehydrogenase rs12676
Male Infertility

Müdaxilə / müalicə

Drug: Betaine supplement

Faza

Faza 1

Qol Qrupları

QolMüdaxilə / müalicə
Experimental: Betaine supplement
Will use powdered betaine (BetaPower, Dupont Nutrition) that is commercially available for food uses. This powder will be delivered as capsules containing 0.5 gram of powdered betaine which will be administered as eleven capsules twice per day (6 in the morning, 5 in the evening) for a daily total of 6 grams of betaine.
Drug: Betaine supplement

Uyğunluq Kriteriyaları

Təhsil üçün uyğun yaşlar 18 Years Üçün 18 Years
Təhsilə Uyğun CinslərMale
Sağlam Könüllüləri qəbul edirBəli
Kriteriyalar

Inclusion Criteria:

- 18 - 60 year old men of multiple races and ethnicities

- Estimated dietary intake of betaine of <150 mg/day

- Carrying two alleles of the rs 12676 single nucleotide polymorphism

Exclusion Criteria:

- Cystathionine-beta-synthase (CBS) deficiency

- Currently taking betaine supplements

- Currently receiving chemotherapy, radiation or any gonadotoxic drug

- Female gender

Nəticə

İlkin nəticə tədbirləri

1. Change in sperm motility from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

Assessed using Computer-Aided Sperm Analysis methodology

2. Change in sperm count from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

3. Change in sperm mitochondrial function from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

Using Seahorse biochemical function assessment

4. Change in sperm ultrastructure from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

Using light and transmission electron microscopy

5. Change in sperm choline dehydrogenase concentration from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

Assessed by Western Blot analysis

6. Change in sperm betaine concentration from baseline [On day zero, day 10, day 30, day 50 and at the end of the 75 day treatment period]

İkincili Nəticə Tədbirləri

1. Betaine intake [At screening and every 21 days during the study]

Assessed using 3-day food records

2. Change in complete blood count from baseline [At 0, 10, 30, 50, and 75 days on treatment]

3. Change in uric acid concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

4. Change in alkaline phosphatase concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

5. Change in aspartate transaminase concentration from baseline [At 0,10, 30, 50, and 75 days on treatment]

6. Change in lactic dehydrogenase concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

7. Change in bilirubin concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

8. Change in blood urea nitrogen concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

9. Change in creatinine concentration from baseline [At 0, 10, 30, 50, and 75 days on treatment]

10. Change in urinalysis parameters from baseline [At 0, 10, 30, 50, and 75 days on treatment]

Facebook səhifəmizə qoşulun

Elm tərəfindən dəstəklənən ən tam dərman bitkiləri bazası

  • 55 dildə işləyir
  • Elm tərəfindən dəstəklənən bitki mənşəli müalicələr
  • Təsvirə görə otların tanınması
  • İnteraktiv GPS xəritəsi - yerdəki otları etiketləyin (tezliklə)
  • Axtarışınızla əlaqəli elmi nəşrləri oxuyun
  • Təsirlərinə görə dərman bitkilərini axtarın
  • Maraqlarınızı təşkil edin və xəbər araşdırmaları, klinik sınaqlar və patentlər barədə məlumatlı olun

Bir simptom və ya bir xəstəlik yazın və kömək edə biləcək otlar haqqında oxuyun, bir ot yazın və istifadə olunan xəstəliklərə və simptomlara baxın.
* Bütün məlumatlar dərc olunmuş elmi araşdırmalara əsaslanır

Google Play badgeApp Store badge