Intestinal Microbiome and Extremes of Atherosclerosis
Açar sözlər
Mücərrəd
Təsvir
Patients were phenotyped by their residual score in linear multiple regression with measured carotid plaque burden as the dependent variable and coronary risk factors were predictors. The residual score essentially represents the distance off the regression line of predicted plaque. They were grouped into three categories: Unexplained atherosclerosis (with more plaque than predicted by risk factors; residual score >2); Explained (the amount of plaque predicted by risk factors, residual score >-2 and <2); and Protected (less plaque than predicted by risk factors, residual score <-2).
DNA was extracted from stool samples in the lab of Dr. Allen-Vercoe at University of Guelph. RNA makeup of the intestinal microbiome was assessed in the lab of Dr. Gregory Gloor at Western. Plasma levels of trimethylamine n-oxide, p-cresylsulfate, hippuric acid, p-cresyl glucuronide, pheny acetyl glutamine and phenyl sulfate were measured by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry in the lab of Dr. Bradley Urquhart at Western.
Nutrient intake over the past year was calculated at the Harvard School of Public Health from the 131 item self-reported and semi-quantitative Harvard Food Frequency Questionnaire (FFQ).
Estimated glomerular filtration rate was calculated from the Chronic Kidney Disease Epidemiological (CKD-EPI) equations.
Tarixlər
| Son Doğrulandı: | 11/30/2017 |
| İlk təqdim: | 12/11/2017 |
| Təxmini qeydiyyat təqdim edildi: | 01/10/2018 |
| İlk Göndərmə: | 01/15/2018 |
| Son Yeniləmə Göndərildi: | 01/10/2018 |
| Son Yeniləmə Göndərildi: | 01/15/2018 |
| Həqiqi Təhsilin Başlama Tarixi: | 06/30/2014 |
| Təxmini İlkin Tamamlanma Tarixi: | 10/14/2016 |
| Təxmini İşin Tamamlanma Tarixi: | 10/14/2016 |
Vəziyyət və ya xəstəlik
Müdaxilə / müalicə
Diagnostic Test: Plasma levels of metabolites
Behavioral: Dietary questionnaire
Diagnostic Test: Intestinal microbiome
Faza
Qol Qrupları
| Qol | Müdaxilə / müalicə |
|---|---|
| Unexplained Atherosclerosis phenotype Residual score in linear regression >2 | |
| Explained Atherosclerosis phenotype Residual score in linear regression <-2, <2 | |
| Protected Atherosclerosis phenotype Residual score <-2 |
Uyğunluq Kriteriyaları
| Təhsil üçün uyğun yaşlar | 18 Years Üçün 18 Years |
| Təhsilə Uyğun Cinslər | All |
| Nümunə götürmə metodu | Probability Sample |
| Sağlam Könüllüləri qəbul edir | Bəli |
| Kriteriyalar | Inclusion Criteria: - Patients attending stroke prevention clinics and a Premature Atherosclerosis Clinic at University Hospital, London, ON, Canada, - with measurements of carotid plaque burden and the risk factors used in the linear regression model. - Willing to consent to the protocol approved by the Ethics board Exclusion Criteria: - Missing data on variables used in the regression model, |
Nəticə
İlkin nəticə tədbirləri
1. Plasma levels of metabolites in the three phenotypes [Day 1]
İkincili Nəticə Tədbirləri
1. Bacterial profile of the intestinal microbiome [Day 1]
2. Effect of nutrient intake on plasma levels of metabolites [Day 1]
3. Effect of renal function on plasma levels of metabolites [Day 1]
