Depression of fast axonal transport produced by tullidora.

The fast axoplasmic transport of labeled proteins was studied in cats showing hindlimb paralysis 4-7 weeks after a single oral dose of tullidora (Karwinskia humboldtiana) toxins. The isotope (3H-leucine) was injected into the spinal ganglion and the contralateral spinal cord of the seventh lumbar segment in order to study transport in sensory and motor fibers. The axoplasmic transport in motor fibers of the sciatic nerve was clearly altered in tullidora-treated cats. The majority of these animals showed a gradual decline of radioactivity from the cord to the periphery instead of the clear-cut wave front always seen in normal cats. An apparent wave was seen in three treated cats but the wave peak was behind the normal position and the slope of the wave front was reduced. While the rate of transport indicated by the farthest extent of the foot of the slope was not in all cases significantly changed, the results all indicated a hindered transport by the reduced slope front in the distal segments of the motor axons. In contrast, the axoplasmic transport appeared normal in the sensory fibers of all but one tullidora-treated cat. Light and electron microscopy of medial gastrocnemius and sural (cutaneous) nerves revealed axonal constrictions and axolemal irregularities associated with organelle retention after tullidora treatment. Also, some mitochondria appeared swollen. These changes were more frequent and intense in the motor nerve fibers than in the cutaneous nerve fibers.