[Study on the flavonoids compounds of Thesium chinese].
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OBJECTIVE
To study the flavonoids compounds from the effective fraction of Thesium chinese in anti-inflammation.
METHODS
The isolation and purification were carried out by many kinds of column chromatographic techniques. The flavonoids were analyzed by HPLC-DAD, and elucidated by 1H-NMR, 13C-NMR and ESI-MS.
RESULTS
Five compounds were obtained and therir structures were determined as kaempferol-3-O-glucoside (I), rutinoside (II), luteolin-7-O-glucoside (III), kaempferyl 5-methyl ether (IV), apigenin-5-O-neohesperidoside (V).
CONCLUSIONS
Compounds IV and V are isolated from this plant for the first time.