Representative biopsy specimens of the removed brain tumor will be sterilely removed and subjected to mechanical trituration and subsequently to enzymatic digestion with collagenase 1 and 3.

Tumor

Brain Tumor

Neuromed IRCCS

Utility of Primary Glioblastoma Cell Lines

Glioblastoma Lines as the Disease Model

Obtaining tumor biopsies to generate primary glioblastoma cell lines. Cells will also be used for phenotypic, genetic (IDH mutation and MGMT promoter methylation) and immunohistochemical characterization (e.g. glial markers GFAP, IDH1, P53, ATRX and proliferation marker Ki67), natural and synthetic drug screening in order to assess a dose-response correlation, or other molecular and cellular techniques.

Antonella Arcella

Principal investigator

Astrocytoma, Grades II, III and IV

Glioblastoma Multiforme

Giant Cell Glioblastoma

Glioma

Oligodendrogliomas

LB-100 will be infused over 2 hours via syringe pump in the OR or 1-12 hours before to surgery. The dose established from a Phase I study will be 2.33 mg/m(2).

LB-100

National Institutes of Health Clinical Center

Protein Phosphatase 2A Inhibitor, in Recurrent Glioblastoma

Phase II Trial of LBlOO, a Protein Phosphatase 2A Inhibitor, in Recurrent Gliomas

To determine the pharmacokinetic properties of LB100 in Gliomas tumor tissues

determine the plasma concentration and calculated PK parameters of LB100 and LB100M (endothall).

determine the concentration LB100 and its major metabolite, 7-oxabicyclo [2.2.1] heptanes-2,3- dicarboxylic acid (LB100M) in glioma tumor tissue when a known non-toxic dose of LB100 is delivered intravenously over 2 hours

Intra-patient PD effect in PBMC and tumor tissue will be evaluated in all subjects for presence of correlation to identify potential predictive markers

determine changes in phospho- protein expression in circulating PBMC

oligodendroglioma/phosphatase

Utility of Primary Glioblastoma Cell Lines

Protein Phosphatase 2A Inhibitor, in Recurrent Glioblastoma

Elm tərəfindən dəstəklənən ən tam dərman bitkiləri bazası