Exercise Induced Changes in Fat Metabolism
Ключавыя словы
Рэферат
Апісанне
General study design
- Subjects who exercise regularly will be instructed to exercise exactly 3 days before the experimental trial, and then abstain from their exercise training program until after the experiment is complete.
- Subjects will arrive to the Substrate Metabolism Laboratory (1210 CCRB) after at least a 6h fast.
- After resting quietly in the laboratory for approximately 30min, Dr. Horowitz will collect a resting blood sample by venipuncture, as he has done in our other IRB approved protocols (e.g., HUM00029179). Briefly, Dr. Horowitz will wash his hands and put on gloves. The participant's skin will be disinfected at the venipuncture site with isopropyl alcohol. Single-use needles will be used for each participant and immediately discarded into a biohazard sharps container after use. After the blood draw, direct pressure will be applied to the site of needle insertion to avoid/reduce bruising and discomfort.
- Subjects will exercise (bike or treadmill) for 1h at a moderate exercise intensity (approximately 65% of their predicted VO2peak). This moderate exercise intensity has been used extensively by Dr. Horowitz's laboratory in previous exercise testing and training studies in both lean and obese individuals, and is well tolerated by both populations.
- During the exercise session, subjects will wear a heart rate monitor to ensure participants are exercising at the correct exercise intensity.
- Subjects will also use the mouthpiece and nose clip (described above) for ~5min at the beginning, middle, and end of exercise to verify they are exercising at approximately 65% of their predicted VO2peak.
- A study team member will be present with the participant at all times during exercise to ensure the comfort and safety.
- Immediately after exercise another blood sample will be collected.
Даты
| Апошняя праверка: | 08/31/2019 |
| Упершыню прадстаўлена: | 10/12/2015 |
| Меркаваная колькасць заявак прадстаўлена: | 10/15/2015 |
| Першае паведамленне: | 10/19/2015 |
| Апошняе абнаўленне адпраўлена: | 09/24/2019 |
| Апошняе абнаўленне апублікавана: | 09/29/2019 |
| Фактычная дата пачатку даследавання: | 09/30/2015 |
| Разліковая дата першаснага завяршэння: | 09/30/2020 |
| Разліковая дата завяршэння даследавання: | 09/30/2022 |
Стан альбо хвароба
Ўмяшанне / лячэнне
Other: Exercise session
Фаза
Групы ўзбраенняў
| Рука | Ўмяшанне / лячэнне |
|---|---|
| Other: Exercise session All subjects will exercise for 1 hour at a moderate intensity. There are no different arms in this study | Other: Exercise session There is no drug or device intervention in this study. Subjects will exercise at a moderate intensity for 60 minutes and a blood sample will be obtained before and after the exercise session |
Крытэрыі прыдатнасці
| Узрост, які мае права на вучобу | 18 Years Каб 18 Years |
| Пол, прыдатны для навучання | All |
| Прымае здаровых валанцёраў | Так |
| Крытэрыі | Inclusion Criteria: - Age: 18-40 - Body Mass Index: 20-35 kg/m2 - Women must have regularly occurring menses and must be premenopausal Exclusion Criteria: - Pregnant or lactating - Evidence/history of cardiovascular or metabolic disease - Medications known to affect lipid or glucose metabolism |
Вынік
Першасныя вынікі
1. changes in plasma concentrations of epinephrine after a session of exercise [1 day]
2. changes in plasma concentrations of lactate after a session of exercise [1 day]
3. changes in plasma concentrations of fatty acids after a session of exercise [1 day]
4. changes in plasma concentrations of IL-6 after a session of exercise [1 day]
5. changes in plasma concentrations of irisin after a session of exercise [1 day]
6. changes in plasma concentrations of growth hormone after a session of exercise [1 day]
