Tetrahydroxystilbene glucoside alleviates Ang Ⅱ-induced senescence of HUVECs via SIRT1

Tetrahydroxystilbene glucoside (TSG), an active ingredient of <i>Polygonum multiflorum</i>, has been known for certain anti-aging effects. In this study, the possible protective mechanism of TSG on human umbilical vein endothelial cells (HUVECs) senescence induced by angiotensin Ⅱ (Ang Ⅱ) was investigated. The results revealed that TSG pretreatment could reduce the percentage of senescence-associated-β-galactosidase (SA-β-gal) positive cells, and decrease the expression levels of the cellular senescence biomarkers, p53 and PAI-1 proteins. At the same time, the expression of SIRT1 in senescent cell showed an upward trend due to TSG treatment. When inhibiting the expression of SIRT1 by EX527, our results showed that TSG reversed the effect of EX527, by promoting the expression level of SIRT1, reducing the expression of SA-β-gal positive cell and the expression level of p53 and PAI-1 proteins. The present study demonstrated that TSG could protect against HUVECs senescence induced by Ang Ⅱ, potentially through modulation of SIRT1 activity.