pipe/phosphatase

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Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes.

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Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were

Reproductive toxicity to male mice of nose only exposure to water- pipe smoke.

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OBJECTIVE Water-pipe smoking (WPS) is popular in the Middle East and is starting to gain popularity in several Western countries as well. It is widely and erroneously perceived to be less harmful than other forms of tobacco use. The reproductive adverse effects of cigarette smoking have been studied

Localization of glucose-6-phosphatase activity in the rat cardiac muscle by cobalt-based cytochemistry.

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The histocytochemical method for the detection of glucose-6-phosphatase activity has been studied in the rat heart tissue by using cobalt-capture technique. The incubation medium had the following ingredients: cobalt chloride, PIPES-NaOH buffer pH = 6.7, glucose-6-phosphate disodium salt and

A three-dimensional observation of acid phosphatase-positive structures in rat testis by high voltage electron microscopy.

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Using a fixative of low concentrated glutaraldehyde in PIPES, we examined three-dimensional structures of acid phosphatase (ACPase) activity in the cells of testes of normal adult and 30 min heat-treated rats by high voltage electron microscopy. There were less ACPase-positive structures in germ

Effects of the amide alkaloid piperyline on apoptosis, autophagy, and differentiation of pre-osteoblasts

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Background: Amide alkaloidsare typical constituents in plants of the Piperaceae family. Most of the pharmacological properties of Piper nigrum L. are attributed to the major amide alkaloid, piperine. Piperyline (PIPE) is a further amide

Buffer, pH, and ionic strength effects on the (Na+ + K+)-ATPase.

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A dog kidney (Na+ + K+)-ATPase preparation also catalyzes K+-independent and K+-activated phosphatase reactions with p-nitrophenyl phosphate as substrate. K+-independent activity increases with declining pH over the range 7.5 to 5.8, whereas the other two activities decrease. The increased

Interaction of tubulin and microtubule proteins with vanadate oligomers.

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Microtubule assembly is known to be regulated by the phosphorylation of microtubule-associated proteins (MAPs), and is thus sensitive to phosphatase inhibitors. We have investigated the direct interaction between phosphatase inhibitors (vanadate, sodium fluoride, and okadaic acid) and microtubule

Antibacterial and osteogenesis performances of LL37-loaded titania nanopores in vitro and in vivo.

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Background: Many studies have shown that the size of nanotube (NT) can significantly affect the behavior of osteoblasts on titanium-based materials. But the weak bonding strength between NT and substrate greatly limits their application. Purpose: The objective of this study was to

Comparative and Interactive Biochemical Effects of Sub-Lethal Concentrations of Cadmium and Lead on Some Tissues of the African Catfish (Clarias gariepinus).

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Cadmium is a strong toxic heavy metal which presents in paints and liquid wastes and causes oxidative stress in fish. On the other hand, lead is widely used for different purposes, e.g. lead pipes, it targets vital organs such as liver and kidney causing biochemical alterations. The present study

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Herbal & Natural Medicine

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