In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines
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StatusZavršeno
Sponzori
Meenakshi Ammal Dental College and Hospital
KLINIČKIM ISPITIVANJIMA: NCT03728192
BioSeek: NCT03728192
Ključne riječi
Sažetak
The present study is an effort to investigate the hypothesis that in-vitro vitality and antiapoptotic effect of alcoholic crude extract of mangosteen on Oral cancer( H357) cell lines and Cevical cancer (HeLa) cell lines.
Opis
The oral and cervical cells were investigated by MTT (3-4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, DNA fragmentation detection by TUNEL (Terminal deoxynucleotidyl transferase-mediated d-UTP Nick End Labeling) assay and Apototic assay using Annexin V/FITC Kit.
Datumi
| Posljednja provjera: | 09/30/2018 |
| Prvo podneseno: | 07/12/2018 |
| Predviđena prijava predata: | 10/29/2018 |
| Prvo objavljeno: | 11/01/2018 |
| Zadnje ažuriranje poslato: | 10/29/2018 |
| Posljednje ažuriranje objavljeno: | 11/01/2018 |
| Stvarni datum početka studija: | 03/06/2016 |
| Procijenjeni datum primarnog završetka: | 05/10/2016 |
| Predviđeni datum završetka studije: | 07/12/2018 |
Stanje ili bolest
Apoptosis
Intervencija / liječenje
Other: Mangosteen treated group
Faza
-
Grupe ruku
| Arm | Intervencija / liječenje |
|---|---|
| Experimental: Mangosteen treated group HeLa and H357 cell lines were procured and were further subdivided into 2 subdivisions and were assigned interventions:
Mangosteen group- cells treated with mangosteen extract and Camptothecin group - cells treated with standard anticancer drug camptothecin(25 micro mole) | Other: Mangosteen treated group For Mangosteen group :DNA fragmentation assay is used to identify apoptosis.1×106 cells were harvested and treated with mangosteen pericarp extract for 48 h For Camptothecin group : Cells were also treated with standard anticancer drug Camptothecin to act as a positive control. |
| No Intervention: Untreated group H357 and HeLa cell line without any drug intervention. |
Kriteriji prihvatljivosti
| Polovi podobni za studiranje | All |
| Prihvaća zdrave volontere | Da |
| Kriterijumi | Inclusion Criteria: - Patients with oral squamous cell carcinoma - Patients with cervical carcinoma Exclusion Criteria: • Patients with leukaemia |
Ishod
Primarne mjere ishoda
1. apoptotic potential of ethanolic extract of mangosteen pericarp on oral and cervical cancer cell lines via apoptotic assay [48 hrs at base line]
Apoptosis assay was performed using Annexin V/FITC Kit (BD Biosciences, Catalog no. 556547), and the fluorescence intensities of FITC-conjugated annexin-V and Propidium iodide (PI) in cells were analyzed using flow cytometry. HeLa and H357 cells (1×106 cells/well) were seeded in a 6-well plate. The cells were allowed to adhere for 12 hrs, cultured in medium containing different concentrations of mangosteen extract for 48hr. The cells were then collected and washed twice with Phosphate buffer Saline, gently resuspended in 100μL annexinV-FITC binding buffer (1x) and incubated with 5μL annexinV-FITC in the dark for 10 min at 25°C. This was followed by centrifugation of cells at 2000 rpm for 5 min, and gently resuspended in 500μL annexinV-FITC binding buffer (1x) and 5μL PI was added in an ice bath, followed by immediate analysis by flow cytometry Cell Quest software (BD Biosciences).
