The Role of Pyroptosis in Chronic Venous Disease
Ključne riječi
Sažetak
Opis
Pyroptosis is a pro-inflammatory form of regulated cell death and is dependent on networks of extracellular fibers (NET,s), on the enzymatic activity of inflammatory proteases that belong to the family of cysteine-dependent aspartate-specific proteases (caspase), and cytokines. The role of inflammation has been previously demonstrated in chronic venous disease (CVD), which is a condition that affects up to 80% of adult population in western countries.
According to the Comprehensive Classification System for Chronic Venous Disorders (CEAP) divided into 6 Clinical stages:
C0: no visible or palpable signs of venous disease. C1: telangiectasies or reticular veins. C2: varicose veins. C3: edema. C4a: pigmentation and eczema. C4b: lipodermatosclerosis and atrophie blanche. C5: healed venous ulcer. C6: active venous ulcer.
The aim of this study is to evaluate the role of pyroptosis in the onset and progression of CVD.
The Investigators will select 200 participants with chronic venous disease (CVD) according to Clinical stage of CEAP (C1-C6 patients) [Group A] . The investigators will also select 200 healthy participants without CVD (C0) as controls [Group B].
Peripheral vein blood samples will be collected from both groups. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated between the two groups and between the subgroups of group A, according to clinical stage.
Datumi
| Posljednja provjera: | 10/31/2018 |
| Prvo podneseno: | 11/11/2018 |
| Predviđena prijava predata: | 11/14/2018 |
| Prvo objavljeno: | 11/15/2018 |
| Zadnje ažuriranje poslato: | 11/14/2018 |
| Posljednje ažuriranje objavljeno: | 11/15/2018 |
| Stvarni datum početka studija: | 11/11/2018 |
| Procijenjeni datum primarnog završetka: | 11/11/2019 |
| Predviđeni datum završetka studije: | 11/11/2020 |
Stanje ili bolest
Intervencija / liječenje
Procedure: Peripheral blood draw
Faza
Grupe ruku
| Arm | Intervencija / liječenje |
|---|---|
| Group A - Participants with CVD Patients with chronic venous disease (CVD) will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples. | |
| Group B - Healthy Participants Voluntary healthy subjects will undergo peripheral blood draw. Markers of pyroptosis (NETs, Caspase-1 and Cytokines) will be evaluated in blood samples. |
Kriteriji prihvatljivosti
| Uzrast podoban za studiranje | 18 Years To 18 Years |
| Polovi podobni za studiranje | All |
| Metoda uzorkovanja | Non-Probability Sample |
| Prihvaća zdrave volontere | Da |
| Kriterijumi | Inclusion Criteria: - Chronic Venous Disease (C1-C6 CEAP classification ) Exclusion Criteria: - Peripheral artery disease - Malignant Tumors - Severe cardiovascular and cerebrovascular disease - Liver and kidney failure |
Ishod
Primarne mjere ishoda
1. Pyroptosis evaluation 1 [2 years]
2. Pyroptosis evaluation 2 [2 years]
3. Pyroptosis evaluation 3 [2 years]
