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cephalomannine/tisa biljka
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Fatal poisoning with Taxus baccata: quantification of paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, cephalomannine (taxol B), and 3,5-dimethoxyphenol in body fluids by liquid chromatography-tandem mass spectrometry.
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This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based
Screening for pharmaceutically important taxoids in Taxus baccata var. Aurea corr. with CC/SPE/HPLC-PDA procedure.
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Needles of 'the golden yew' Taxus baccata var. Aurea Corr. were extracted with methanol followed by pre-purification of the crude extract and column chromatographic (CC) separation on florisil in gradient mode (an increasing concentration of acetone in dichloromethane). The obtained fractions were
Immunoenzymatic methods applied to the search for bioactive taxoids from Taxus baccata.
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Polyclonal antibodies raised against 2'-succinyltaxol-bovine serum albumin (BSA) conjugate were used for the immunodetection of bioactive taxoids in chromatographic fractions of the stem bark extract of Taxus baccata. In addition to taxol, cephalomannine, and baccatin III, two taxoids were isolated
Eight cases of fatal and non-fatal poisoning with Taxus baccata.
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This paper describes two fatalities, three non-fatal intentional and three accidental oral ingestions of yew (Taxus baccata) leaves. In all cases the post-mortem external examinations showed no signs of violence. Internal examinations revealed small green, needle-like particles on the tongue, in the
Seasonal changes in the concentrations of four taxoids in Taxus baccata L. during the autumn-spring period.
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The concentrations of four common taxoids: baccatin III, paclitaxel, cephalomannine and 10-deacetylbaccatin III (10-DAB III) were measured in fresh needles and stems of Taxus baccata L. during the late autumn-spring period (November'96-April'97) which has not been investigated to date in this
Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC-MS/MS.
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BACKGROUND
Taxus baccata L. is an evergreen conifer whose plant parts are cardiotoxic. Only the red arils of the berries are described as non-toxic and taxane-free.
OBJECTIVE
Extraction and HPLC-MS/MS methods were developed for the investigation of the Taxus compounds 3,5-dimethoxyphenol,
Taxol on the surface of leaves and inside the needles of three species and two varieties of Taxus.
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Leaves of three outside-growing Taxus species (Taxus baccata L.; T. baccava var. elegantissima; T cuspidata Sieb, et Zucc; T. media var. Hatfieldii; T media var. Hickii) and two varieties contained 26-890 μg/g of taxol (paclitaxel®), thus are substantial renewable resources for obtaining this drug.
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