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Analysis of glycoalkaloids from potato shoots and tomatoes by four-sector tandem mass spectrometry with scanning-array detection: comparison of positive ion and negative ion methods.
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A wide range of glycoalkaloids from potato shoots and tomatoes, including trisaccharide-containing glycoalkaloids (alpha-chaconine, alpha-solanine, and alpha-solasonine), tetrasaccharide-containing glycoalkaloids (alpha-tomatine and demissine), and disaccharide-containing glycoalkaloids (beta
Enhancing monoterpene alcohols in sweet potato shochu using the diglycoside-specific β-primeverosidase.
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Monoterpene alcohols (MTAs) are characteristic flavour-imparting compounds in sweet potato shochu (Japanese distilled spirit) that are liberated following hydrolysis by specific enzymes during fermentation. In the present study, we evaluated the effect of an exogenously added diglycoside-specific
Structural studies of the carbohydrate moieties of lectins from potato (Solanum tuberosum) tubers and thorn-apple (Datura stramonium) seeds.
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1. Methylation analysis of potato (Solanum tuberosum) lectin and thorn-apple (Datura stramonium) lectin confirmed previous conclusions that both glycoproteins contained high proportions of l-arabinofuranosides and lesser amounts of d-galactopyranosides. The arabinofuranosides are present in both
Sugar response element enhances wound response of potato proteinase inhibitor II promoter in transgenic tobacco.
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The promoter region of the potato proteinase inhibitor II (PI-II) gene was studied to identify cis-acting regulatory sequences involved in sugar response using transgenic tobacco plants. The 5' control region covering an 892 nucleotide sequence upstream from the cap site and a 32 nucleotide
Analysis of subcellular metabolite levels of potato tubers (Solanum tuberosum) displaying alterations in cellular or extracellular sucrose metabolism.
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The expression of a heterologous invertase in potato tubers (Solanum tuberosum) in either the cytosol or apoplast leads to a decrease in total sucrose content and to an increase in glucose. Depending on the targeting of the enzyme different changes in phenotype and metabolism of the tubers occur:
A rapid, sensitive, and cost-efficient assay to estimate viability of potato cyst nematodes.
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Potato cyst nematodes (PCNs) are quarantine organisms, and they belong to the economically most relevant pathogens of potato worldwide. Methodologies to assess the viability of their cysts, which can contain 200 to 500 eggs protected by the hardened cuticle of a dead female, are either time and
[Measuring of the energy turnover in adult swine during feeding of rations with potato starch, potatoes, beets, pressed shreds and coarse fodder as supplements to a basic ration. 2. Evaluation and discussion of the results for further development of energetic feed rating for swine].
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Energy metabolism measurings of male castrates of a live weight between 90 and 180 kg after feeding rations with a wide variety in the content of various carbohydrates (crude fibre 36-185, water soluble carbohydrates 25-306, starch 272-683 g/kg DM) formed the experimental basis of the
Biovar of Ralstonia solanacearum Causing Bacterial Wilt of Tomato in Nigeria.
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A survey of southwestern Nigeria showed an outbreak of wilt disease caused by Ralstonia solanacearum in 80% of tomato (Lycopersicon lycopersicum) fields in the production area of Ogun State (7°15'N, 3°25'E) in June 1996. Subsequent surveys conducted in Edo (6°45'N, 5°30'E), Delta (5°15'N, 5°45'E),
Kinetic modelling of acrylamide formation during the finish-frying of french fries with variable maltose content.
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In light of a recent update in EU regulations governing levels of acrylamide in foodstuffs, further understanding of the role of different precursors is fundamental to extending mitigation strategies into a wider product range. Kinetic modelling was used to investigate the role of maltose in the
Characterization of seven basic endochitinases isolated from cell cultures of Citrus sinensis (L.).
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Seven endochitinases (EC 3.2.1.14) (relative molecular masses 23,000-28,000 and isoelectric points 10.3-10.4) were purified from nonembryogenic Citrus sinensis L. Osbeck cv. Valencia callus tissue. The basic chitinase/lysozyme from this tissue (BCLVC) exhibited lysozyme, chitinase and chitosanase
Cellulase-assisted extraction of oligosaccharides from defatted rice bran.
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Defatted rice bran was subjected to cellulase treatment in order to increase its extractable oligosaccharides. Various combinations of enzyme concentration (0%, 0.5%, 1.0%, and 2.0%), temperature (room, 30, 40, and 50 degrees C), and time (1, 3, 5, and 16 h) were tested to identify the optimum
A polymer with a backbone of 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonic acid, a teichuronic acid, and a beta-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124.
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Structures of cell wall anionic polymers of the strain Streptomyces sp. VKM Ac-2124, a causative agent of potato scab, which is phylogenetically the closest to plant pathogenic species S. setonii and S. caviscabies, were studied. The strain contains three anionic glycopolymers, viz., a teichuronic
[Anionic polymers of the cell wall of Streptomyces sp. VKM An-2534].
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The cell wall of Streptomyces sp. VKM An-2534, the causative agent of common scab in potato tubers, which does not synthesize thaxtomin and is phylogenetically close to phytopathogen Streptomyces setonii sp. ATCC 25497, contains two anionic carbohydrate-containing polymers. The major polymer is
Generation of organic acids and monosaccharides by hydrolytic and oxidative transformation of food processing residues.
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Carbohydrate-rich biomass residues, i.e. sugar beet molasses, whey powder, wine yeast, potato peel sludge, spent hops, malt dust and apple marc, were tested as starting materials for the generation of marketable chemicals, e.g. aliphatic acids, sugar acids and mono-/disaccharides. Residues were
Replacement of digestible by resistant starch lowers diet-induced thermogenesis in healthy men.
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The present study describes the effect of replacement of digestible starch by resistant starch (RS) on diet-induced thermogenesis (DIT), postprandial glucose and insulin responses, and colonic fermentation. Ten healthy males consumed three test meals, consisting of diluted, artificially-sweetened
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