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giardiasis/glutation
Veza se sprema u međuspremnik
Page 1 od 25 rezultati
Effect of resin disinfectants-I3 and -I5 on Giardia muris and Giardia lamblia.
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The resin-I5 column developed in our laboratories rendered aqueous suspensions containing up to 5 X 10(4) cysts of Giardia muris or Giardia lamblia per ml incapable of excystation. The inhibition of excystation was effective at both 4 and 25 degrees C. The addition of Na2S2O3 to column eluates
Glutathione and trypanothione in parasitic hydroperoxide metabolism.
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Thiol-dependent hydroperoxide metabolism in parasites is reviewed in respect to potential therapeutic strategies. The hydroperoxide metabolism of Crithidia fasciculata has been characterized to comprise a cascade of three enzymes, trypanothione reductase, tryparedoxin, and tryparedoxin peroxidase,
Factors promoting in vitro excystation of Giardia muris cysts.
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Giardia muris cysts, isolated from mouse faeces, excysted routinely at levels greater than 90%, when induced in 1X Hanks' supplemented with 17 mM glutathione, 29 mM L-cysteine-HCl, and 50 mM NaHCO3 for 30 minutes at 35 degrees C, followed by washing and suspension in trypsin-Tyrode's solution at pH
The monothiol single-domain glutaredoxin is conserved in the highly reduced mitochondria of Giardia intestinalis.
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The highly reduced mitochondria (mitosomes) of Giardia intestinalis are recently discovered organelles for which, it was suggested, iron-sulfur cluster assembly was their only conserved function. However, only an incomplete set of the components required for FeS cluster biogenesis was localized to
Plasmid vectors for proteomic analyses in Giardia: purification of virulence factors and analysis of the proteasome.
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In recent years, proteomics has come of age with the development of efficient tools for purification, identification, and characterization of gene products predicted by genome projects. The intestinal protozoan Giardia intestinalis can be transfected, but there is only a limited set of vectors
A thioredoxin reductase-class of disulphide reductase in the protozoan parasite Giardia duodenalis.
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We describe the purification and characterisation of a thioredoxin reductase-like disulphide reductase from the ancient protozoan parasite, Giardia duodenalis. This dimeric flavoprotein contains 1 mol FAD per subunit and had an apparent subunit molecular mass of 35 kDa. The purified enzyme catalysed
Transcriptional profiling of Giardia intestinalis in response to oxidative stress.
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Giardia intestinalis is a microaerophilic parasite that infects the human upper small intestine, an environment that is fairly aerobic with reactive oxygen species being produced to fight off the parasite. It is quite perplexing how Giardia, lacking conventional eukaryotic antioxidant machinery
Antioxidant defences of Spironucleus vortens: Glutathione is the major non-protein thiol.
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The aerotolerant hydrogenosome-containing piscine diplomonad, Spironucleus vortens, is able to withstand high fluctuations in O₂ tensions during its life cycle. In the current study, we further investigated the O₂ scavenging and antioxidant defence mechanisms which facilitate the survival of S.
Antioxidant defence systems in the protozoan pathogen Giardia intestinalis.
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The microaerophilic protist Giardia intestinalis is the causative agent of giardiasis, one of the most common intestinal infectious diseases worldwide. The pathogen lacks not only respiratory terminal oxidases (being amitochondriate), but also several conventional antioxidant enzymes, including
Interaction of Giardia intestinalis and Systemic Oxidation in Preschool Children in the Western Highlands of Guatemala.
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Guatemala is a country with the highest prevalence of stunting in under-5 children in the Americas, with a national average of 49.8%. Asymptomatic intestinal colonization with Giardia intestinalis is common in Guatemalan preschoolers and has been implicated as a factor in linear growth retardation.
Differential gene expression in Giardia lamblia under oxidative stress: significance in eukaryotic evolution.
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Giardia lamblia is a unicellular, early branching eukaryote causing giardiasis, one of the most common human enteric diseases. Giardia, a microaerophilic protozoan parasite has to build up mechanisms to protect themselves against oxidative stress within the human gut (oxygen concentration 60 μM) to
Anaerobic bacterial metabolism in the ancient eukaryote Giardia duodenalis.
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The protozoan parasite, Giardia duodenalis, shares many metabolic and genetic attributes of the bacteria, including fermentative energy metabolism which relies heavily on pyrophosphate rather than adenosine triphosphate and as a result contains two typically bacterial glycolytic enzymes which are
Lactobacillus rhamnosus GG antagonizes Giardia intestinalis induced oxidative stress and intestinal disaccharidases: an experimental study.
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The present study describes the in vivo modulatory potential of Lactobacillus rhamnosus GG (LGG), an effective probiotic, in Giardia intestinalis-infected BALB/c mice. Experimentally, it was observed that oral administration of lactobacilli prior or simultaneous with Giardia trophozoites to mice,
Infectivity of Giardia duodenalis Assemblages A and E for the gerbil and axenisation of duodenal trophozoites.
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Establishing in vitro cultures of Giardia duodenalis trophozoites from faecal cysts remains very difficult due to poor excystation and bacterial contamination. This study investigated an alternative approach starting from duodenal trophozoites of gerbils that were artificially infected with field
Antioxidant defense mechanisms in parasitic protozoa.
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Many of the parasitic protozoa, such as Entamoeba histolytica, Giardia, Trypanosoma, Leishmania, and Plasmodium, are considered to be anaerobes because they can be grown in vitro only under conditions of reduced oxygen tension. However, these parasitic protozoa have been found to be aerotolerant or
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