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l homocysteine/hypoxia
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Adrenergic blockade blunts adenosine concentration and coronary vasodilation during hypoxia.
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Myocardial hypoxia is thought to be an important stimulus for increasing interstitial adenosine concentration. The adenosine hypothesis of coronary control was investigated during steady-state hypoxia by making measurements of coronary venous and epicardial well adenosine concentrations in
Adenosine metabolism in the guinea pig heart: the role of cytosolic S-adenosyl-L-homocysteine hydrolase, 5'-nucleotidase and adenosine kinase.
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This study was conducted to elucidate the role of S-adenosyl-L-homocysteine (SAH) hydrolase, 5'-nucleotidase and adenosine kinase in the production and removal of adenosine in the isolated guinea pig heart during normoxic (95% O2) and hypoxic (30% O2) perfusion. Using an adenosine kinase inhibitor
Synthesis and radiobiological applications of [35S]L-homocysteine thiolactone.
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[35S]L-Homocysteine thiolactone ([35S]L-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the
Hypoxia in the renal medulla: implications for hydrogen sulfide signaling.
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Hydrogen sulfide (H(2)S) is enzymatically generated in mammalian tissues from either L-cysteine or L-homocysteine. H(2)S possesses multiple biological activities, including regulation of vascular tone and blood pressure. Hydrogen sulfide produced in endothelial cells, vascular smooth muscle cells,
The transmethylation pathway as a source for adenosine in the isolated guinea-pig heart.
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In order to quantify adenosine production from the transmethylation pathway [S-adenosylmethionine (AdoMet)----S-adenosylhomocysteine (AdoHcy) in equilibrium adenosine + L-homocysteine] in the isolated guinea-pig heart under basal conditions (normoxic perfusion with 95% O2) and during elevated
Formation of S-adenosylhomocysteine in the heart. I: An index of free intracellular adenosine.
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To assess the concentration of free intracellular adenosine in the heart the kinetic properties of cytosolic S-adenosylhomocysteine (SAH) hydrolase were utilized at elevated levels of L-homocysteine (adenosine + L-homocysteine in equilibrium with SAH + H2O). Global hypoxia was induced in the
Role of S-adenosylhomocysteine hydrolase in adenosine metabolism in mammalian heart.
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S-Adenosylhomocysteine hydrolase of mammalian hearts from different species is exclusively a cytosolic enzyme. The apparent Km for the guinea-pig enzyme was 2.9 microM (synthesis) and 0.39 microM (hydrolysis). Perfusion of isolated guinea-pig hearts for 120 min with L-homocysteine thiolactone (0.23
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