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omega 3 fatty acid/duhan
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Two low-temperature-inducible Chlorella genes for delta12 and omega-3 fatty acid desaturase (FAD): isolation of delta12 and omega-3 fad cDNA clones, expression of delta12 fad in Saccharomyces cerevisiae, and expression of omega-3 fad in Nicotiana tabacum.
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In an attempt to clarify the involvement of fatty acid desaturases (FADs) in the freezing tolerance of Chlorella vulgaris IAM C-27, developed by hardening, we have isolated cDNA clones for two types of FADs from the Chlorella strain, based on the sequence information of genes for delta12 and omega-3
Cloning of a cDNA encoding tobacco omega-3 fatty acid desaturase.
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A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast omega-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those
Enhancement of α-linolenic acid content in transgenic tobacco seeds by targeting a plastidial ω-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER.
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CONCLUSIONS
Expression of sesame plastidial FAD7 desaturase modified with the endoplasmic reticulum targeting and retention signals, enhances the α-linolenic acid accumulation in seeds of Nicotiana tabacum. In plants, plastidial ω-3 fatty acid desaturase-7 (FAD7) catalyzes the formation of C16 and
cDNA cloning of a wounding-inducible gene encoding a plastid omega-3 fatty acid desaturase from tobacco.
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A cDNA encoding the plastid omega-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins.
Genetic Enhancement of Cold Tolerance by Expression of a Gene for Chloroplast [omega]-3 Fatty Acid Desaturase in Transgenic Tobacco.
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The increased production of trienoic fatty acids, hexadecatrienoic (16:3) and linolenic (18:3) acids, is a response connected with cold acclimation of higher plants and is thought to protect plant cells against cold damage. Transgenic tobacco (Nicotiana tabacum cv SR1) plants that contain increased
Enhanced cold tolerance in transgenic tobacco expressing a chloroplast omega-3 fatty acid desaturase gene under the control of a cold-inducible promoter.
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A new cold-inducible genetic construct was cloned using a chloroplast-specific omega-3-fatty acid desaturase gene (FAD7) under the control of a cold-inducible promoter (cor15a) from Arabidopsis thaliana. RT-PCR confirmed a marked increase in FAD7 expression, in young Nicotiana tabacum (cv. Havana)
Chronic ozone exacerbates the reduction in photosynthesis and acceleration of senescence caused by limited N availability in Nicotiana sylvestris.
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Both elevated ozone (O(3)) and limiting soil nitrogen (N) availability negatively affect crop performance. However, less is known about how the combination of elevated O(3) and limiting N affect crop growth and metabolism. In this study, we grew tobacco (Nicotiana sylvestris) in ambient and elevated
Enhanced sensitivity to higher ozone in a pathogen-resistant tobacco cultivar.
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Investigations of the effects of elevated ozone (O(3)) on the virus-plant system were conducted to inform virus pathogen management strategies better. One susceptible cultivar of tobacco (Nicotiana tabacum L. cv. Yongding) and a resistant cultivar (Nicotiana tabacum L. cv. Vam) to Potato virus Y
Characterization of transgenic tobacco with an increased alpha-linolenic acid level
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Microsomal omega-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (alpha-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes. Transgenic tobacco (Nicotiana tabacum) plants with increased 18:3 contents (designated SIIn
How the redox state of tobacco 'Bel-W3' is modified in response to ozone and other environmental factors in a sub-tropical area?
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This study intended to determine whether the redox state in plants of Nicotiana tabacum 'Bel-W3' fluctuates in response to the environmental factors in a sub-tropical area contaminated by ozone (São Paulo, SE-Brazil) and which environmental factors are related to this fluctuation, discussing their
Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent Pseudomonas syringae Pathovars.
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We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide ((*)NO) production from tobacco (Nicotiana tabacum). LPAD calibration against (*)NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection
Effect of ozone on swelling of tobacco mitochondria.
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The swelling of mitochondria isolated from leaves, roots, and callus tissues of Nicotiana tabacum, L, var. White Gold, was measured by following changes in optical density at 520 mmu in buffered 0.25 m sucrose or 0.125 m KCl. Ozone induced rapid swelling of the isolated mitochondria and increased
The efficiency of tobacco Bel-W3 and native species for ozone biomonitoring in subtropical climate, as revealed by histo-cytochemical techniques.
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We aimed to verify whether hydrogen peroxide (H(2)O(2)) accumulation and cell death are detected early in three bioindicators of ozone (O(3)), Nicotiana tabacum 'Bel-W3', Ipomoea nil 'Scarlet O'Hara' and Psidium guajava 'Paluma', and whether environmental factors also affect those microscopic
Comparison of the Substrate Preferences of ω3 Fatty Acid Desaturases for Long Chain Polyunsaturated Fatty Acids.
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Omega-3 long chain polyunsaturated fatty acids (ω3 LC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3) are important fatty acids for human health. These ω3 LC-PUFAs are produced from their ω3 precursors by a set of desaturases and elongases involved in the
Origin of Bel-W3, Bel-C and Bel-B tobacco varieties and their use as indicators of ozone.
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Since 1962, the tobacco variety Bel-W3 (Nicotiana tabacum L.), has been used in many countries as an indicator of the presence of phytotoxic concentrations of O(3). It is super-sensitive to O(3) and may produce easily recognizable symptoms for several weeks on the new, fully expanded leaves. Bel-B
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