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Anticardiolipin antibodies and dependence of a serum cofactor. A mechanism of thrombosis.
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OBJECTIVE
To evaluate the dependence on the serum cofactor of anticardiolipin antibodies (aCL) in infectious and autoimmune diseases. We also studied their correlation with some clinical manifestations, specially thrombosis.
METHODS
aCL were determined with a standard ELISA method, and a modified
[Studies of the etiology of infectious abortion in cows in Ruse District].
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A total of 28,159 blood serum samples have been examined for brucellosis, 87--for leptospirosis, 84--for toxoplasmosis, 554--for the presence of rickettsii and neoreckettsii, and 193--for total protein content, albumin, and protein fractions, taken from cows in the course of the years on 40 dairy
Contamination of commercial PCR master mix with DNA from Coxiella burnetii.
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Contamination of an in-house diagnostic real-time PCR for Q fever was traced back to a commercially obtained PCR Master Mix. It was established that this Master Mix contained DNA from Coxiella burnetii, probably as a result of the use of compounds of animal origin such as bovine serum albumin.
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