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ribonucleic acid/kukuruz
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Characterization of ribonucleic acid synthesis by nuclei isolated from Zea mays.
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Nuclei were isolated from the shoots of Zea mays and assayed for endogenous RNA polymerase activity in vitro. Maximum incorporation from radioactive precursors (70 pmol [(3)H]uridine 5' monophosphate/100 μg DNA) was reached after incubation for 1 h at 25°C. The RNA product, analysed by
Effects of Potassium Nutrition on Ribonucleic Acid and Ribonuclease in Zea mays L.
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Potassium deficiency decreased the total RNA per shoot in corn (Zea mays L.) seedlings due to the reduced sizes of the plant, but increased the ratio of RNA to dry matter as much as 40%. Total base composition of RNA was unaffected by K(+) deficiency. Thus K(+) deficiency does not appear to alter
[Contamination of soluble ribonucleic acids of immature seeds of Zea mays by a phenol-carboxylic acid].
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A rapid technique for the estimation of polynucleotide adenylyltransferase and ribonucleic Acid polymerase in plant tissues.
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Nucleic acid-dependent polynucleotide adenylytransferase (EC 2.7.7.19) and ribonucleic acid polymerase (EC 2.7.7.6) have been partially purified from maize tissues (Zea mays L.) utilizing ammonium sulfate precipitation and batch diethylaminoethylcellulose chromatography. The technique is applicable
Effect of light on ribonucleic Acid metabolism in greening maize leaves.
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The effect of illumination on the incorporation of labeled precursors into RNA of dark-grown maize (Zea mays) leaves was studied using either (32)P-phosphate or double labeling with (14)C- and (3)H-uridine. In the dark, label was preferentially incorporated into etioplast ribosomal RNAs.
Estradiol increases amounts of messenger ribonucleic acid for gonadotropin-releasing hormone receptors in sheep.
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Two experiments were conducted simultaneously to investigate regulation of amounts of mRNA for GnRH receptors during the periovulatory period in sheep. In the first experiment, amounts of mRNA for GnRH receptors were measured before and after preovulatory surge of LH following regression of the CL
Up-regulation of oxytocin receptor messenger ribonucleic acid and protein by estradiol in the cervix of ovariectomized rat.
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Oxytocin receptor (OTR) regulation has been extensively studied in uterine myometrium and endometrium. However, studies in the cervix are limited. The present studies utilized in situ hybridization and immunocytochemistry to localize OTR mRNA and protein distribution in cervices of nonpregnant
Sequence of Reactivation of Ribonucleic Acid Synthesis during Early Germination of the Maize Embryo.
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The onset of RNA synthesis in primary roots of germinating Zea mays embryos was studied. Incubation of excised embryos in the radioactive precursor solution was performed after different germination periods.It was observed that there are three successive stages in the reactivation of RNA synthesis.
Maize mitochondria: purification and characterization of ribosomes and ribosomal ribonucleic Acid.
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Mitochondria were prepared from etiolated maize shoots (Zea mays L. var. McNair 508) by homogenization followed by differential centrifugation and equilibrium banding in discontinuous sucrose or Renografin-sucrose gradients. Mitochondria prepared by sucrose banding showed better physiological
Changes in hepatic lipid parameters and hepatic messenger ribonucleic acid expression following estradiol administration in laying hens (Gallus domesticus).
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Fatty liver hemorrhagic syndrome (FLHS) is characterized by increased hepatic triacylglycerol content associated with liver hemorrhages and results in a sudden decline in egg production. Genetic, environmental, nutritional, and hormonal factors have all been implicated in the etiology of FLHS, but
Expression and inducibility of aryl hydrocarbon receptor pathway genes in wild-caught killifish (Fundulus heteroclitus) with different contaminant-exposure histories.
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Wildcaught killifish from a contaminated site on the Elizabeth River (VA, USA) are refractory to induction of cytochrome P4501A (CYP1A, measured as catalytic activity and immunodetectable CYP1A protein) after exposure to typical aryl hydrocarbon receptor (AHR) agonists, as has been reported for fish
An Immunity (QL3) Breaking Strain of Sorghum Mosaic Potyvirus in India.
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Sorghum (Sorghum bicolor) line Tx2786 is immune to strains or isolates of sorghum mosaic potyvirus (SrMV strains SCH and SCH) but susceptible to strains of maize dwarf mosaic (MDMV) and sugarcane mosaic (SCMV) potyviruses (1,2). When grown in proximity to sugarcane, Tx2786 was infected with a virus
Quantitation of the bioenergetics of a tuberculosis infection in chicks.
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Interaction of an avian tuberculosis infection with a known metabolizable energy yield of dietary corn oil in chicks was used to quantitate total host energy expenditure necessitated by the infectious process. Three trials in which two doses of inoculum were used resulted in mild and severe
Sequestration of 2,3,7,8-tetrachlorodibenzo-p-dioxin by activated carbon eliminates bioavailability and the suppression of immune function in mice.
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The effectiveness of activated carbon in reducing the bioavailability of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was examined from the context of using in situ sorbent amendments to remediate soils/sediments contaminated with polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs). This
In Vitro Synthesis of a Precursor to the Methionine-rich Polypeptide of the Zein Fraction of Corn.
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The messenger ribonucleic acid fraction isolated from a protein bodyenriched fraction of developing corn (Zea mays L.) endosperm stimulated the incorporation of radioactive amino acids into at least five polypeptides when added to a wheat germ extract capable of protein synthesis. Of these, the two
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