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solanum jamesii/nikotin
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Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events.
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Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. The use of the incompletely homologous (homeologous) Solanum plastid DNA as donor resulted in a Nicotiana
Molecular analysis of the stylar-expressed Solanum chacoense small asparagine-rich protein family related to the HT modifier of gametophytic self-incompatibility in Nicotiana.
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Gametophytic self-incompatibility (GSI) systems involving the expression of stylar ribonucleases have been described and extensively studied in many plant families including the Solanaceae, Rosaceae and Scrophulariaceae. Pollen recognition and rejection is governed in the style by specific
A multiple stress-responsive gene ERD15 from Solanum pennellii confers stress tolerance in tobacco.
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Wild species often show more tolerance to environmental stress factors than their cultivated counterparts. An early responsive-to-dehydration gene was cloned from a drought- and salt-tolerant wild tomato Solanum pennellii (SpERD15). SpERD15 transcript accumulated differentially in different organs,
Structural transitions in viroid-like RNAs associated with cadang-cadang disease, velvet tobacco mottle virus, and Solanum nodiflorum mottle virus.
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The conformational transitions of viroid-like RNAs associated with cadang-cadang disease, velvet tobacco mottle virus, and solanum nodiflorum mottle virus were studied by melting analysis and fast temperature jump technique in 1 mM sodium-cacodylate, 10 mM NaCl, 0.1 mM EDTA, pH 6.8. The 4 circular
Studies on encapsidated viroid-like RNA I. Characterization of velvet tobacco mottle virus.
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Velvet tobacco mottle virus (VTMoV) isolated from Nicotiana velutina growing wild in arid Central Australia was transmitted by inoculation to a limited number of plant species of which N. clevelandii was the most convenient experimental host. The virus was also transmitted from field-grown plants
Chemical and serological comparison of the coat proteins of velvet tobacco mottle and Solanum nodiflorum mottle viruses.
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Velvet tobacco mottle virus (VTMoV) and Solanum nodiforum mottle virus (SNMV) are serologically closely related as shown by immunodiffusion tests. Dissociated VTMoV coat protein separates into three electrophoretic components in polyacrylamide gels corresponding to polypeptides with Mr's of about
Studies on encapsidated viroid-like RNA. III. Comparative studies on RNAs isolated from velvet tobacco mottle virus and Solanum nodiflorum mottle virus.
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Solanum nodiforum mottle virus (SNMV) encapsidates two unique RNA species as shown by 3H-complementary DNA hybridization analysis. They are RNA 1, a long linear single-stranded molecule with a molecular weight of 1.5 x 10(6) and RNA 2 and RNA 3 which are circular and linear forms of the same
Studies on encapsidated viroid-like RNA. IV. Requirement for infectivity and specificity of two RNA components from velvet tobacco mottle virus.
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Neither the virus-like RNA (RNA 1) nor the viroid-like RNA (RNA 2) of velvet tobacco mottle virus (VTMoV) is capable of independent replication in Nicotiana clevelandii. The function of RNA 2 for the RNA 1 replication could not be fulfilled by conventional viroids such as those of chrysanthemum
Hatch and Reproduction of Globodera tabacum tabacum in Response to Tobacco, Tomato, or Black Nightshade.
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The effects of broadleaf tobacco, tomato, and black nightshade on juvenile hatch and reproduction of Globodera tabacum tabacum were determined in laboratory and greenhouse experiments. Root exudates from nightshade stimulated greater egg hatch than those from either 'Rutgers' tomato or '86-4'
Trap Crops and Population Management of Globodera tabacum tabacum.
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Tobacco, eastern black nightshade, and tomato were grown for 3 to 13 weeks to assess differences in invasion, development, and soil density of Globodera tabacum tabacum (tobacco cyst nematode) in field plots and microplots over three seasons. Tobacco cyst nematodes invaded roots of resistant and
Carcinogens and endemic squamous cancer of the oesophagus in Transkei, South Africa. Environmental initiation is the dominant factor; tobacco or other carcinogens of low potency or concentration are sufficient for carcinogenesis in the predisposed mucosa.
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Oesophageal cancer is common in Africa, and is a major cause of death in Transkei, South Africa. The cause of this endemic disease has been debated for many decades. A continuing focus of research has been identification of a single potent carcinogen. I argue that endemic incidence of oesophageal
Rapid, random evolution of the genetic structure of replicating tobacco mosaic virus populations.
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The effects of temperature and type of host on the evolution of variants in replicating populations of tobacco mosaic virus (TMV) originating from an in vitro RNA transcript of a cDNA clone were studied. The phenotypic markers measured were temperature-sensitive (ts) replication and induction of
SpPKE1, a Multiple Stress-Responsive Gene Confers Salt Tolerance in Tomato and Tobacco.
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Understanding the mechanism of abiotic-tolerance and producing germplasm of abiotic tolerance are important in plant research. Wild species often show more tolerance of environmental stress factors than their cultivated counterparts. Genes from wild species show potential abilities to improve
The SbASR-1 gene cloned from an extreme halophyte Salicornia brachiata enhances salt tolerance in transgenic tobacco.
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Salinity severely affects plant growth and development. Plants evolved various mechanisms to cope up stress both at molecular and cellular levels. Halophytes have developed better mechanism to alleviate the salt stress than glycophytes, and therefore, it is advantageous to study the role of
Development of a simple sample preparation technique for gas chromatographic-mass spectrometric determination of nicotine in edible nightshades (Solanaceae).
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The purpose of this study was to develop a rapid analytical method for the reliable determination of low concentrations of nicotine in foods for large numbers of samples. Food material was extracted using a simple liquid-liquid extraction method. For processed foods, further clean-up steps had to be
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