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Page 1 od 16 rezultati
Structure of grafted crown-gall teratoma shoots of tobacco: Regulation of transformed cells.
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Crown-gall teratomas are tumors of higher plants with an intrinsic capacity for organogenesis. The growth pattern of tobacco (Nicotiana tabacum L.) teratoma shoots, which is highly aberrant in primary tumors, becomes normal when the shoots are grafted to healthy stock plants. However, certain
Auxin-facilitated utilization of glutamic acid by tobacco crown-gall teratoma cells.
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Teratoma tissues obtained by inoculating Nicotiana tabacum cv. "Turkish" with a moderately virulent strain of the crown-gall bacterium require the synthetic auxin, α-naphthaleneacetic acid (NAA) when glutamic acid is used as a sole nitrogen source in the culture medium. In contrast, growth on
Temperature-sensitive expression of auxin-autotrophy by crown-gall teratoma cells of tobacco.
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Crown-gall teratoma tissues of tobacco (Nicotiana tabacum L.) grow in culture at 25° in the absence of added auxin or cell-division factors. The capacity of these tissues to grow without added auxin is temperature sensitive. At 35° auxin provided as α-naphthalene acetic acid or
Tumor and teratoma induction in tobacco plants by debudding.
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Stem and root tumors and teratomata may be induced in tobacco plants (Nicotiana tabacum L., var. One Sucker) by total debudding. Intact plants or plants only decapitated produced no tumors or teratomata throughout their life time. These findings suggest a possibly important oncological relationship
Auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of Agrobacterium tumefaciens.
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We have studied the mechanism of auxin autonomy in tobacco (Nicotiana tabacum L.) crowngall tissues transformed by the auxin-mutant (tms (-)) A66 strain of Agrobacterium tumefaciens. Normally, tms (-) tobacco tumor tissues require the formation of shoots to exhibit auxin-independent growth in
Inhibitory effects of elevated endogenous cytokinins on nitrate reductase in ipt-expressing tobacco are eliminated by short-term exposure to benzyladenine.
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Using a novel system for expressing ipt gene from Agrobacterium tumefaciens in tobacco (Nicotiana tabacum L., cv. Petit Havana SR1), we were able to grow seedlings and teratoma-like tissue with increased content of cytokinins. This material enabled us to investigate new regulatory aspects of nitrate
Acid phosphatase activity of normal and tumor tissue of tobacco grown in vitro and in vivo.
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Tissue cultures of Nicotiana labacum consisting of green, albino and habituated (normal origin) and teratoma (tomorous origin) were grown under asceptic conditions for 6 to 8 weeks and their extracts were analyzed for phosphatase activity. Comparative enzyme analyses were also made on crude stem
Superoxide dismutase and peroxidase are coordinately regulated in differentiated and transformed tissues of Nicotiana tabacum.
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We used a series of normal and Agrobacterium-transformed, bacteria-free tobacco tissue cultures which differ in their levels of histodifferentiation to test the relationship of superoxide dismutase, peroxidase, and catalase to oncogenic transformation and differentiation. When compared with normal
The relationship of indole-3-acetic acid content and growth of crown-gall tumor tissues of tobacco in culture.
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We have measured the content of the auxin, indole-3-acetic acid (IAA), in cloned, crown-gall teratoma line of Nicotiana tabacum L. cv. "Turkish" by a highly specific and sensitive radioimmunoassay. This tissue line, which does not require auxin for continuous growth in culture, exhibits two phases
Morphogenic responses of debudded tobacco plants to gibberellic Acid and indole-3-acetic Acid.
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Stem applications of indole-3-acetic acid (IAA) or gibberellic acid (GA) did not prevent or alter tumor or teratoma formation in debudded tobacco plants (Nicotiana tabacum L., var. One Sucker). The materials produced intense (in case of GA) and moderate (in case of IAA) stem proliferations when
Hormonal control of tobacco crown gall tumor morphology.
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The endogenous levels of auxin and cytokinin in teratoma and unorganized tobacco (Nicotiana tabacum L. var Wisconsin #38) crown gall tumor tissues were determined. Teratoma tissues contain levels of auxin and cytokinin favorable for shoot formation, whereas unorganized tumors contain levels of auxin
Independent integration and seed-transmission of the TR-DNA of the octopine Ti plasmid pTi Ach5 in Nicotiana plumbaginifolia.
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After co-cultivation of diploid Nicotiana plumbaginifolia protoplasts with an octopine-type Agrobacterium tumefaciens strain (LBA 4013) putative transformants were selected for hormone-independent growth, and were tested for T-DNA markers. The number of transformants expressing only TL-DNA markers,
Tumorigenesis inheritance from the putative progenitor species of Nicotiana rustica.
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Hybrid seedlings from crosses of Nicotiana rustica×N. langsdorffii and N. rustica×N. alata show tumors including teratomas and vitrification. In the present study, we attempted to elucidate the genetic background leading to tumorigenesis and vitrification from the
Neoplastic progression in crown gall in tobacco without elevated auxin levels.
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We have isolated two stable variants from a crown-gall teratoma tissue of tobacco (Nicotiana tabacum L.) transformed by Agrobacterium tumefaciens strain A66, a mutant of the virulent A6 strain containing an insertion sequence in the tumor-inducing (Ti) plasmid at the locus coding for auxin
[Molecular genetics and biotechnology in medicinal plants: studies by transgenic plants].
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The advances in molecular genetics and biotechnology in the field of medicinal plant research are discussed with focusing on the works using transgenic plants. Differentiated organ cultures and transgenic teratomas, incited by the infection with mutants of Agrobacterium Ti and Ri plasmids, were
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