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Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase from papaver somniferum plant cell cultures. The enzyme catalyzing the formation of thebaine in morphine biosynthesis.
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Acetyl coenzyme A:salutaridinol-7-O-acetyltransferase, a highly substrate-specific enzyme, has been purified nearly 3,000-fold to homogeneity from Papaver somniferum plant cell suspension cultures. Purification was achieved by fractionated ammonium sulfate precipitation, dye-ligand affinity
Thebaine from root cultures of Papaver bracteatum.
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Root cultures derived from cell suspension cultures of Papaver bracteatum were shown to produce thebaine (yield 0.03%).
Colorimetric determination of thebaine in Papaver bracteatum.
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A rapid colorimetric test for the determination of thebaine in dried, extracted Papaver bracteatum is described. The method is based on the development of an orange color formed by the nitroso color reaction for phenols. The absorption peak is near 450 nm. The linear range response is from 0 to 1.0
Papaver bracteatum Lindl.: quantitative extraction and determination of thebaine.
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An interlaboratory procedure for the quantitative extraction and analysis of thebaine from different tissues of Papaver bracteatum Lindl. is presented. Each step was evalutated for the yield of thebaine by use of 1-3H-thebaine and GLC. The method of drying and milling of tissue and the size of
[Determination of thebaine in various tissues of papaver bracteatum by gas chromatography].
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Papaver bracteatum is an important source of opiates because of its high thebaine content. An efficient and convenient method of determining thebaine, not only in the root, but in the more highly pigmented capsule, stem and leaf, is urgently required. We decided to examine these in detail on a wide
Quantitative determination of thebaine in Papaver bracteatum by high-pressure liquid chromatography.
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A method is described for the quantitative analysis of thebaine from Papaver bracteatum, using a single high-pressure liquid chromatographic column. The procedure gives base-line separation of thebaine without the need for gradient elution equipment, and can be completed within 12 min. Thebaine
The effects of selected fungicides and insecticides on growth and thebaine production of Papaver bracteatum.
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The fungicides benlate, maneb, and captan depressed plant growth, thebaine concentration, and thebaine yield per plant when administered chronically to Papaver bracteatum. The chronic administration of dicofol inhibited plant growth and thebaine yield per plant, but did not affect thebaine
Plant regeneration and thebaine content of plants derived from callus culture of Papaver bracteatum.
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Plants have been regenerated from embryogenic callus cultures of two varieties of Papaver bracteatum and successfully transplanted to soil. Regeneration occurred in good yield, around 40 plants to soil within 5 months per 0.4g piece of original callus. Thebaine concentrations comparable to those in
Genetic improvement of Papaver bracteatum: heritability and selection response of thebaine and Seed Yields.
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The heritability and repeatability of various characters affecting thebaine and seed yields in Papaver bracteatum were estimated from parents and progeny families during two consecutive years of growth. Thebaine and seed yields per plant showed the lowest heritability and therefore low selection
Rapid semi-quantitative spot test for determination of thebaine and differentiation of Papaver bracteatum from P. orientale and P. pseudo-orientale.
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The alkaloids, orientalidine, oripavine, thebaine, narceine, narceine, codeine, papaverine, isothebaine, alpenigenine and morphine react with a solution of sulfuric acid containing molybdate to give specific colors. Using the acid solution permitted the differentiation of Papaver bracteatum latex
Direct spectrophotometric determination of thebaine in Arya II population capsules of Papaver bracteatum Lindl.
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A simple spectrophotometric determination of thebaine based on the complexation reaction with bromcresol green was developed. The yellow complex was extracted with chloroform over the pH 1.5--4.5 range. The solution of the complex in chloroform showed the maximum absorption at 415 nm and obeyed
Accumulation and Distribution of Thebaine in the Roots of Papaver bracteatum During Plant Development.
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The thebaine-yield components in the roots of PAPAVER BRACTEATUM were studied at various stages of plant development. The maximum concentration of thebaine was obtained at the start of flowering. However the dry weight of the roots and the thebaine yield increased until full flowering. Significant
The determination of thebaine in Papaver bracteatum Lindl by gas-liquid chromatography.
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Several solvents have been examined in the process of developing a convenient method for extracting powdered material and removing interfering pigments to produce an extract of Papaver bracteatum suitable for g.l.c. assay. The most suitable extracting solvent was aqueous acetic acid (5%); the assay
Thebaine variations between germplasm sources within one collection of Papaver bracteatum Lindl.
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Plants of Papaver bracteatum Lindl. derived from different germplasm sources within one Iranian collection were grown for 18 and 37 weeks in the greenhouse and controlled environment chambers. Gas-chromatographic analyses showed significant differences between thebaine concentrations of root tissue
Increase in thebaine content of Papaver bracteatum Lindl. after colchicine treatment of seeds. Annual fluctuations in thebaine level of individual plants.
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From colchicine-treated seeds of Papaver bracteatum Lindl. some poppy plants were obtained that developed capsules richer in thebaine than the controls. The individual poppies were analyzed for capsule thebaine content annually for eight successive years, the results revealing significant
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