We describe glycosyltransferase nucleic acids and proteins isolatable from Arabidopsis thaliana, transgenic cells expressing said glycosyltransferases, and bioreactors comprising said transgenic cells together with nutrient medium for supporting the growth of said cell that includes at least one exogenous substrate which is a substrate for said glycosyltransferase, and wherein said nutrient medium does not include an exogenous supply of UDP-glucose.

The University of York

Bioreactor containing cells expressing glycosyltransferase nucleic acids isolatable from Arabidopsis

The present invention is directed to compositions and methods related to the synthesis and modification of uridine-5'-diphospho-sulfoquinovose (UDP-SQ). In particular, the methods of the present invention comprise the utilization of recombinant enzymes from Arabidopsis thaliana, UDP-glucose, and a sulfur donor to synthesize UDP-SQ, and the subsequent modification of UDP-SQ to form compounds including, but not limited to, 6-sulfo-.alpha.-D-quinovosyl diaclyglycerol (SQDG) and alkyl sulfoquinovoside. The compositions and methods of the invention provide a more simple, rapid means of synthesizing UDP-SQ, and the subsequent modification of UDP-SQ to compounds including, but not limited to, SQDG.

udp glucose/arabidopsis thaliana

Bioreactor containing cells expressing glycosyltransferase nucleic acids isolatable from Arabidopsis

Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ)

Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ)

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