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yellow fever/carbohydrate
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Exchanging the yellow fever virus envelope proteins with Modoc virus prM and E proteins results in a chimeric virus that is neuroinvasive in SCID mice.
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A chimeric flavivirus infectious cDNA was constructed by exchanging the premembrane (prM) and envelope (E) genes of the yellow fever virus vaccine strain 17D (YF17D) with the corresponding genes of Modoc virus (MOD). This latter virus belongs to the cluster of the "not-known vector" flaviviruses and
Characterization of yellow fever virus proteins E and NS1 expressed in Vero and Spodoptera frugiperda cells.
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The cDNA encoding the E and NS1 proteins of the yellow fever virus (YFV) was expressed in Spodoptera frugiperda cells via the recombinant baculovirus Ac-E. NS1 as a gp100 precursor which was cleaved to generate the recombinant proteins E and NS1 similar in size, folding and antigenicity to the
Nutritional Quality during Development Alters Insulin-Like Peptides' Expression and Physiology of the Adult Yellow Fever Mosquito, Aedes aegypti.
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Mosquitoes have distinct developmental and adult life history, and the vectorial capacity of females has been shown to be affected by the larval nutritional environment. However, little is known about the effect of developmental nutrition on insulin-signaling and nutrient storage. In this study, we
Comparative immunochemical and biological analysis of African and South American yellow fever viruses.
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Four geographic variants (topotypes) of yellow fever (YF) virus from Africa and South America previously determined by RNA oligonucleotide mapping were analyzed for their structural, antigenic and virulence characteristics. The electrophoretic migration mobility and carbohydrate content of the
Failure of yellow fever immunization to produce a catabolic response in individuals fully adapted to a protein-sparing modified fast.
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In previous studies, patients consuming a protein-sparing modified fast (PSMF) did not develop the expected increase in nitrogen excretion following incidental infectious illness. To assess the catabolic response with a controlled infection in such patients, 17-D yellow fever vaccine was
A derivate of the antibiotic doxorubicin is a selective inhibitor of dengue and yellow fever virus replication in vitro.
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A doxorubicin derivate, SA-17, that carries a squaric acid amide ester moiety at the carbohydrate (α-l-daunosaminyl) group was identified as a selective inhibitor of in vitro dengue virus (DENV) serotype 2 replication (50% effective concentration [EC(50)] = 0.34 ± 0.20 μg/ml [0.52 ± 0.31 μM]). SA-17
Pathogenesis and Inhibition of Flaviviruses from a Carbohydrate Perspective.
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Flaviviruses are enveloped, positive single stranded ribonucleic acid (RNA) viruses with various routes of transmission. While the type and severity of symptoms caused by pathogenic flaviviruses vary from hemorrhagic fever to fetal abnormalities, their general mechanism of host cell entry is
Heterogeneity in envelope protein sequence and N-linked glycosylation among yellow fever virus vaccine strains.
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We have compared the deduced envelope (E) protein sequences of two biologically well-characterized yellow fever (YF) virus vaccine strains. The 17DD strain has been produced in Brazil for more than 50 years and used to successfully vaccinate millions of people worldwide. The 17D-213 is a candidate
Flight performance and teneral energy reserves of two genetically-modified and one wild-type strain of the yellow fever mosquito Aedes aegypti.
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The ability of sterile males to survive, disperse, find, and mate with wild females is key to the success of sterile insect technique (SIT). The Release of Insects carrying a Dominant Lethal (RIDL) system is a genetics-based SIT strategy for Aedes aegypti. We examine two aspects of insect
Microorganism-Based Larval Diets Affect Mosquito Development, Size and Nutritional Reserves in the Yellow Fever Mosquito Aedes aegypti (Diptera: Culicidae).
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Background
Mosquito larvae feed on organic detritus from the environment, particularly microorganisms comprising bacteria, protozoa, and algae as well as crustaceans, plant debris, and insect exuviae. Little attention has been paid to nutritional studies in Aedes aegyptiImpacts of Dietary Nutritional Composition on Larval Development and Adult Body Composition in the Yellow Fever Mosquito ( Aedes aegypti)
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Background: the mosquito Aedes aegypti (Ae. aegypti) is an important vector of arboviruses, including Zika, Dengue, and Chikungunya. The dietary requirements of larval Ae. aegypti are not well understood and likely
Comparative proteome analysis reveals that blood and sugar meals induce differential protein expression in Aedes aegypti female heads.
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Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein
Genetically modified viruses: vaccines by design.
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Vaccination has been one of the most successful and cost-effective health interventions ever employed. One disease (smallpox) has been eradicated, another (poliomyelitis) should disappear early in the new millennium and a third (measles) should follow shortly after. Conventional vaccines usually
Larvicidal activity of lectins from Myracrodruon urundeuva on Aedes aegypti.
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Aedes aegypti transmits etiologic agents of yellow fever and dengue. Vaccine for dengue virus is not available and vector control is essential to minimize dengue incidence. This report deals with the larvicidal activity of lectins isolated from Myracrodruon urundeuva bark (MuBL) and heartwood
Why do female Aedes aegypti (Diptera: Culicidae) feed preferentially and frequently on human blood?
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Adult female Aedes aegypti (L.), the vector of dengue and yellow fever viruses, have an affinity for feeding on human blood and a tendency to forego feeding on sugar. This observation challenges two tenets of mosquito biology: (1) mosquitoes imbibe plant carbohydrates for synthesis of energy
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