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The maize bHLH transcription factor bHLH105 confers manganese tolerance in transgenic tobacco.
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Manganese (Mn) toxicity is an important limiting factor for crop production in acidic soils. The basic helix-loop-helix (bHLH) transcription factors are involved in a variety of physiological processes. However, whether the bHLHs are involved in excess Mn stress response is largely unknown. Here, we
Linear molecules of tobacco ptDNA end at known replication origins and additional loci.
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Higher plant plastid DNA (ptDNA) is generally described as a double-stranded circular molecule of the size of the monomer of the plastid genome. Also, the substrates and products of ptDNA replication are generally assumed to be circular molecules. Linear or partly linear ptDNA molecules were
Changes in chloroplast DNA during development in tobacco, Medicago truncatula, pea, and maize.
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We examined the DNA from chloroplasts obtained from young and fully expanded leaves of tobacco (Nicotiana tabacum L.), Medicago truncatula, pea (Pisum sativum L.), and maize (Zea mays L.). The changes in plastid DNA content and structure were monitored by four independent methods:
Ectopic terpene synthase expression enhances sesquiterpene emission in Nicotiana attenuata without altering defense or development of transgenic plants or neighbors.
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Sesquiterpenoids, with approximately 5,000 structures, are the most diverse class of plant volatiles with manifold hypothesized functions in defense, stress tolerance, and signaling between and within plants. These hypotheses have often been tested by transforming plants with sesquiterpene synthases
Characterization of auxin-binding protein 1 from tobacco: content, localization and auxin-binding activity.
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There is evidence that auxin-binding protein 1 (ABP1) is an auxin receptor on the plasma membrane. Maize (Zea mays L.) possesses a high level of auxin-binding activity due to ABP1, but no other plant source has been shown to possess such an activity. We have analyzed the ABP1 content of tobacco
Effects of benzo(a)pyrene and nicotine on prostaglandin synthesis in buccal pouch and submandibular glands of the Syrian hamster.
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Adult male Syrian hamsters were treated by swabbing the apex of the buccal pouch with corn oil (control, C), 1 mM benzo(a)pyrene (BP), nicotine (NC), or BP+NC in corn oil, twice daily, 5 days a week. After a 4-week treatment, the pouches and submandibular glands were dissected and used for the
Stomatal Behavior and Water Status of Maize, Sorghum, and Tobacco under Field Conditions: II. At Low Soil Water Potential.
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Diurnal changes in the vertical profiles of irradiance incident upon the adaxial leaf surface (I), leaf resistance (r(1)), leaf water potential (psi), osmotic potential (pi), and turgor potential (P) were followed concurrently in crops of maize (Zea mays L. cv. Pa602A), sorghum (Sorghum bicolor [L.]
Tobacco ringspot virus Found in the Cardboard Cycad (Zamia furfuracea) in Florida.
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Zamia furfuracea (Zamiaceae) is native of coastal Mexico. It is a popular houseplant and easy to grow outdoors in warm climates. In November 2005, a plant of Z. furfuracea, originally from Texas, was received at the Division of Plant Industry in Gainesville, FL. The plant had numerous chlorotic
Isolation and Characterization of a 295-bp Strong Promoter of Maize High-Affinity Phosphate Transporter Gene ZmPht1; 5 in Transgenic Nicotiana Benthamiana and Zea Mays
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The small 295-bp ZmPht1; 5 promoter is sufficient to drive high-intensity expression of target genes, especially under phosphate deprivation conditions, and is therefore useful for crop improvement via transgenic techniques. Phosphate (Pi) deficiency has become a major challenge and limiting factor
Recycling of Solanum sucrose transporters expressed in yeast, tobacco, and in mature phloem sieve elements.
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The plant sucrose transporter SUT1 (from Solanum tuberosum, S. lycopersicum, or Zea mays) exhibits redox-dependent dimerization and targeting if heterologously expressed in S. cerevisiae (Krügel et al., 2008). It was also shown that SUT1 is present in motile vesicles when expressed in tobacco cells
Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants.
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To test the feasibility of altering polyamine levels by influencing their catabolic pathway, we obtained transgenic tobacco (Nicotiana tabacum) plants constitutively expressing either maize (Zea mays) polyamine oxidase (MPAO) or pea (Pisum sativum) copper amine oxidase (PCuAO), two extracellular and
ZmMKK4 regulates osmotic stress through reactive oxygen species scavenging in transgenic tobacco.
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Mitogen-activated protein kinase kinase (MAPKKs) are important components of MAPK cascades, which are universal signal transduction modules and play important role in regulating both plant development and biotic or abiotic stress responses. In this study, we identified the group C MAPKK gene,
Molecular heterogeneity of photosystem I. psaD, psaE, psaF, psaH, and psaL are all present in isoforms in Nicotiana spp.
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The protein composition of photosystem I (PSI) was examined in Nicotiana spp. by high-resolution polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and immunoblot analysis. Five PSI proteins show polymorphism in an amphidiploid species, Nicotiana tabacum, but not in its ancestral
Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays).
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Aldehyde dehydrogenases (ALDHs) play a central role in detoxification processes of aldehydes generated in plants when exposed to the stressed conditions. In order to identify genes required for the stresses responses in the grass crop Zea mays, an ALDH (ZmALDH22A1) gene was isolated and
Overexpression of the phosphatidylinositol synthase gene from Zea mays in tobacco plants alters the membrane lipids composition and improves drought stress tolerance.
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Phosphatidylinositol (PtdIns) is an important lipid because it serves as a key membrane constituent and is the precursor of the inositol-containing lipids that are found in all plants and animals. It is synthesized from cytidine-diphosphodiacylglycerol (CDP-DG) and myo-inositol by PtdIns synthase
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