Density and mineral content dynamics of bone tissue of the lower jaw of the rat on the background of opioid influence and after its withdrawal.

Introduction: Over the past few years, the use of pharmaceuticals by the population, in particular, analgesics, has become widespread, and often their use is unreasonable from the medical point of view and leads to structural and functional changes in organs and systems of the body and to the development of drug dependence. The aim of our work was to study the qualitative changes and dynamics of the mineral content of rat bone tissue under prolonged opioid exposure.Materials and methods: The study was performed on 40 sexually mature male rats with a body weight of 180-200 g and 3.5 months old. Opioid dependence was modeled by the daily (once a day at the same time interval) injection of a narcotic analgetic Nalbuphine.Results and conclusions: The study showed that the density and mineral content of the bone tissue of the rat's mandible on the background of 6 weeks opioid action of nalbuphine and after its withdrawal have a pronounced dynamics, different and characteristic for each of the investigated parameters. The results of the atomic absorption spectral analysis of the bone tissue of the rat's mandible body allow the determination of the quantitative content of four macro-elements (Ca, P, Mg, Na) and four microelements (K, Fe, Sr, Zn) and investigate their dynamics during the experiment. On the background of the nalbuphine use absolute indices of calcium, phosphorus, magnesium, sodium, zinc and iron content increase, remaining above the norm after its withdrawal; the amount of potassium decreases and remains lower than norm and after the withdrawal of nalbuphine, and the amount of strontium is also reduced, but minimal, and after the withdrawal of nalbuphine has the same value as in intact animals.