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Revised Methods for Purification of Ribosyl-trans-zeatin from Vinca rosea L. Crown Gall Tumor Tissue.
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Ribosyl-trans-zeatin has been purified from Vinca rosea L. crown gall tumor tissue by using two new sequences of isolation procedures. Identification of the compound has been established by mass spectrometry, ultraviolet absorbancy spectra, chromatographic values, and growth activity. The isolation
Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced Nitrogen.
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Several compounds containing reduced nitrogen markedly increased the yields of cell-division compounds extractable from an A6 Vinca rosea L. crown gall tumor tissue. Casein hydrolysate, several amino acids, and ammonium salts were effective. Both trans-zeatin and ribosyl-trans-zeatin were
Uptake of potassium ions by normal and crown-gall-tumor cells of Vinca rosea grown in tissue culture.
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Potassium uptake in normal and crown-gall tissue culture cells of Vinca rosea L. (Cathalanthus roseus G. Don.) was studied. When grown on White's medium for 14-20 d, the tumor cells had a higher K(+) content that did the normal cells. At this logarithmic stage of growth, the rate of K(+) uptake was
Cell-division factors from Vinca rosca L. crown gall tumor tissue.
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A cell-division factor has been precipitated from extracts of cultured Vinca rosea L. crown gall tumor tissue by using the mercuric acetate procedure previously employed by Wood and colleagues to obtain their "cytokinesin I." On the basis of its mass spectrum, ultraviolet light absorbancy spectra,
The structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells.
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The ability of two compounds, a cytokinin and an auxin, to stimulate tobacco cell growth and differentiation has been known for greater than 30 years, but the molecular mechanism of this activation is still unknown. Previous reports of factors endogenous in crown gall tumors of Vinca rosea that
Ribosyl-trans-Zeatin, A Major Cytokinin Produced by Crown Gall Tumor Tissue.
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A cell division factor in crystalline form has been isolated from ethanolic extracts of cultured Vinca rosea L. crown gall tumor tissue. The crystalline material shows the melting point, mass spectrum, ultraviolet-light absorbancy spectra, solubilities, chromatographic migration values, ion-exchange
VINCRISTINE IN THE TREATMENT OF NEOPLASTIC DISEASE.
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Seventy-eight patients with advanced malignant disease were treated with vincristine, an alkaloid derived from Vinca rosea Linn. Fifty-nine of these survived one month from the beginning of treatment and could be evaluated. Twenty made a good response, with return to activity and more than 75%
Quantitative estimation of Agrobacterium tumefaciens DNA in crown gall tumor cells.
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Several reports suggest that Agrobacterium tumefaciens nucleic acids can induce transformation of the cells of susceptible host plants and that bacteria-free tissue cultures of transformed cells contain A. tumefaciens DNA, RNA, antigens, or bacteriophages. We assayed Vinca rosea tumor DNA for base
A Transformation-induced Alteration of Cellular Membranes in Crown Gall Tumor Cells.
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Phospholipids were utilized as a membrane marker to test for transformation-induced alteration of cellular membranes of cultured crown gall cells of Vinca rosea L. Fully transformed cells contained less than half the amount of phospholipids (7.8 micrograms lipid P per gram fresh weight) of normal V.
Revised identification of the chromophore of a cell division factor from crown gall tumor cells of Vinca rosea L.
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Low-resolution mass spectrometry, UV spectra in acid, neutral, and alkaline solution, and nuclear magnetic resonance indicated that the chromophore of one member of a new class of cell division-promoting factors isolated from crown gall tumor tissues consisted of a 3,7-alkyl-2-alkylthio-6-purinone
Glucosyl Zeatin and Glucosyl Ribosylzeatin from Vinca rosea L. Crown Gall Tumor Tissue.
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Recently detected but unidentified cytokinin activity in crown gall tumor tissue from Vinca rosea L. grown on media containing sources of reduced nitrogen has now been attributed to two adenine-type cytokinins. These compounds are glucopyranosyl derivatives of zeatin and ribosylzeatin. The
Vincristine promotes migration and invasion of colorectal cancer HCT116 cells through RhoA/ROCK/ Myosin light chain pathway.
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Vincristine is an antitumor vinca alkaloid isolated from vinca rosea, and is a medication used to treat a number of types of cancer. In this study, we investigated the impact of vincristine on oncogenic phenotypes of human colorectal cancer HCT116 cells. MTT assay demonstrated that vincristine
Therapeutic applications of medicinal plants in the treatment of breast cancer: a review of their pharmacology, efficacy and tolerability.
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Various active compounds (or their semi-synthetic derivatives) derived from medicinal plants have been assessed for their efficacy and tolerability in the treatment of breast cancer. Some of these plant species, including Taxus baccata (paclitaxel, docetaxel), Podophyllum peltatum (etoposide),
[Cisplatin and vinca alkaloid combination chemotherapy of advanced non-small-cell lung cancer in the aged].
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Fifteen patients aged over 65 years of age with advanced non-small-cl lung cancer (mean age = 70.7, stage IIIb: IV = 4:11) were treated with combination chemotherapy consisting of Cisplatin (50 or 80 mg/m2) and a vinca-alkaloid (Vindesine 3 mg/m2 or Etoposide 80 mg/m2). The effectiveness and side
Effective combination therapy of metastatic murine solid tumors with edatrexate and the vinca alkaloids, vinblastine, navelbine and vindesine.
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Studies are described in which a new folate analogue, edatrexate (EDX), in combination with the vinca alkaloids, vinblastine (VBL), navelbine (NVB) or vindesine (DVA) was evaluated against E0771 mammary adenocarcinoma, T241 fibrosarcoma and the Lewis lung tumor. Each of the four agents when given
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