Anticancer activity of Neptunia oleracea methanolic extracts

Neptunia oleracea Lour (water mimosa) is an edible medicinal plant used in treating various diseases. According to Phytochemical and Ethnobotanical Databases, Neptunia oleracea Lour is used in curing earaches, dysentery, syphilis, and tumour. The present study was aimed at demonstrating the anticancer activity of the Neptunia oleracea Lour methanolic extract. The methanolic extract was isolated and its anti-proliferative activity was studied on haematological cancer cell lines. The activity of the extract was further evaluated using cell cycle analysis and apoptosis assays. In addition to this, effect of the extract on c-Myc and PErk1/2 modulation was also evaluated. Neptunia oleracea Lour extract induced cell death in cancer cells while sparing normal cells. An increase in cleaved PARP and reduction in BCL-2 levels observed upon treatment. Neptunia oleracea causes reduction in c-Myc levels and pERK1/2 protein levels. Thus, our work highlights the methanolic extract of Neptunia oleracea Lour as a promising anti-cancer agent.

Keywords: Neptunia oleracea Lour; PARP; c-Myc; cell cycle analysis; fabaceae; leukemia; lymphoma.