Strana 1 z 17 Výsledek
RNA was extracted from spleens of diarrhea and non-diarrhea piglets of Jiuyang Pig, Jianbai Pig and Landrace. RNA pools were established and DDRT-PCR using three anchored and seven arbitrary primers combined with silver staining was conducted to identify ESTs differentially expressed in diarrhea
BACKGROUND
Diarrheal disease is a major cause of morbidity and mortality in humans and animals, including non human primates. While the diagnostics for gastrointestinal bacterial and parasitic pathogens and their etiological role in disease are well established, little is known about the
Rotaviruses were detected using ELISA and latex agglutination, in 210 of 826 (25.4%) stool specimens collected from children with gastroenteritis between October 1984 and August 1990. In 82 of the 85 specimens (96%), Rotavirus specific RNA pattern was detected with polyacrylamide gel electrophoresis
(A) A blue fringe along the surface epithelium of the colonic mucosa on hematoxylin-eosin staining, a characteristic histology of intestinal spirochetosis. (B) Numerous spirochetes highlighted on Warthin-Starry silver staining.
Typical Pneumocystis carinii pneumonia was found in a male thoroughbred foal (46 days old) suffering from diarrhea and hypogammaglobulinemia after birth. The characteristics of the organisms were demonstrated by Grocott methenamine silver staining, and scanning and transmission electron microscopy.
An outbreak of acute gastroenteritis in a kibbutz in southern Israel, characterized by diarrhea, fever, vomiting, and abdominal pain, involved 32 kibbutz members of all ages. Nineteen percent of the children and 3.5% of the adults were ill. Transmission of the illness occurred in direct proportion
Human rotaviruses (HRV) are the most important etiologic agents of acquired diarrhea in infants and young children worldwide. Therefore, the early diagnosis is essential for effective patient management and infection control. We have developed a rapid, simple technique for the diagnosis of rotavirus
Rotaviruses are the most important etiological agents of severe diarrheal illness in infants, children, and adults throughout the world. Group A rotavirus causes approximately 40% of hospitalization for diarrhea among under 5 years children. The prevalence of Group B rotaviruses is not as high as
The incidence and RNA electropherotypes of rotavirus in stools or rectal swabs of children with diarrhea were studied for three rotavirus seasons (1981 through 1984) in Philadelphia, Pa. We used a simplified RNA analysis method involving polyacrylamide gel electrophoresis followed by silver
Rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. Group A rotavirus causes approximately 40% of hospitalization for diarrhea among under 5 years children. Polyacrylamide gel electrophoresis (PAGE) and silver staining was applied to detect rotavirus
Fifty Escherichia coli strains belonging to nine classic EPEC (enteropathogenic E. coli) O:H serotypes from infantile diarrhea were examined for enzyme polymorphism and typed according to their multilocus genotypes. They were further examined for lipopolysaccharide (LPS) patterns by sodium dodecyl
A purification scheme was developed that allowed for the partial purification of complete, double-shelled particles of the infectious diarrhea of infant rats (IDIR) virus, a group B rotavirus (GBR). Structural proteins from the isolated, complete viral particles were separated by sodium dodecyl
BACKGROUND
A 56-year-old female with a 30-year history of ileocolic Crohn's disease presented with a 1-month history of bloody diarrhea and decreased caliber of stools; physical examination revealed a broad indurated anal fissure. The patient had been receiving antimetabolite therapy with
Diarrhea is one of the most common problem of public health worldwide, specially in developing countries. In Venezuela, this affection must be weekly reported when it occurs in children under five years of age. During June 1993 to May 1995, 379 stool specimens were obtained from children under five
Diffuse ileal thickening and ileocecocolic lymphadenomegaly were observed during exploratory laparotomy in a 2-year-old male Japanese snow macaque (Macaca fuscata) that had flu-like signs and diarrhea. Cytologic examination of ileal biopsy imprints revealed many mature, mildly karyolytic neutrophils