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12 Výsledek
Role of sialic acid in selective silver staining of red cell glycophorins.
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Přihlášení Registrace
A causal relationship between the presence of sialic acid and erythrocyte sialoglycoprotein-specific silver staining (Dzandu et al. 1984) has been examined. Quantitative chemical hydrolysis of sialic acid resulted in total loss of sialoglycoprotein-specific staining suggesting a causal role for
Projection lines and the ipsilateral retino-geniculate pathway in the hooded rat.
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Přihlášení Registrace
The organization of the hooded rat's dorsal lateral geniculate nucleus was studied with anatomical techniques, with particular regard to the representation of temporal retina and the binocular field. The ipsilateral and contralateral retinal terminal fields were examined in three stereotaxic planes
The diencephalic course of regenerating retinotectal fibres in Xenopus tadpoles.
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Přihlášení Registrace
The normal retinotectal path in the diencephalon of Xenopus tadpoles is widely distributed in the form of a wedge of fibres extending from the central grey to the outer margin of the diencephalon. Regenerating optic nerve fibres were shown, by silver-staining and proline autoradiography, to follow
Procyclin: an unusual immunodominant glycoprotein surface antigen from the procyclic stage of African trypanosomes.
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Přihlášení Registrace
An immunodominant species-specific surface glycoprotein antigen was purified from procyclic culture forms of Trypanosoma brucei rhodesiense using lectin affinity chromatography and a monoclonal antibody immunoadsorbent. The purified molecule appears on a 10% polyacrylamide gel as a wide, dark silver
Growth, synthesis and regional specialization of the embryonic chicken lens capsule.
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Přihlášení Registrace
The growth, synthesis and regional specialization of the lens capsule has been studied in chicken embryos and compared to adult chickens and mammals. During the final 15 days of embryonic development the surface area of the capsule increased 11-fold. This represents the minimum estimate of capsule
Structural and functional studies on the human C3b/C4b receptor (CR1) purified by affinity chromatography using a monoclonal antibody.
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Přihlášení Registrace
A procedure was devised that has several advantages over previously described methods to purify CR1 from both erythrocytes (E) and the HL-60 promyelocytic cell line. Using a monoclonal antibody immunoaffinity column, CR1 was purified to homogeneity as assessed by silver staining and 2-D gel
Isolation and amino acid composition of the isotypes of a rat Clara cell specific protein.
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Přihlášení Registrace
A protein of molecular weight about 10,000 (Clara cell protein C) present in lung lavage fluid and specific to Clara cells has been shown to have three isotypes. The isotypes have been individually isolated and purified by a combination of molecular sieving, ion exchange chromatography, column
Identification, assay, and purification of a Cdc2-activating threonine-161 protein kinase from human cells.
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Přihlášení Registrace
The biological activities of cyclin-dependent, proline-directed protein kinases (PDPKs) are highly regulated by a complex series of protein phosphorylation/dephosphorylation reactions involving both catalytic and regulatory subunits. In this paper we report on the enzymatic activation of
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.
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Přihlášení Registrace
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution
PROTEOMICS ANALYSIS OF OVEREXPRESSED PLASMA PROTEINS IN RESPONSE TO COLD ACCLIMATION IN Ostrinia furnacalis.
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Přihlášení Registrace
Many insects in temperate regions overwinter in diapause. In these insects, one of the metabolic adaptations to cold stress is the synthesis of responsive proteins. Using proteomic analysis, an investigation aimed to a better understanding of the molecular adaptation mechanisms to cold stress was
Isolation and characterization of rat sperm tail outer dense fibres and comparison with rabbit and human spermatozoa using a polyclonal antiserum.
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Přihlášení Registrace
Rat outer dense fibres were isolated from cauda epididymal spermatozoa using mechanical and chemical dissection methods. Sperm tail isolation procedures were monitored by phase-contrast microscopy and the purity of the outer dense fibres was verified by electron microscopy. SDS-PAGE of isolated
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins.
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Přihlášení Registrace
The proteins in human parotid saliva have been separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis into 20 or more well resolved species. The Coomassie Brilliant Blue (CBB) R-250 and silver staining procedures have been modified to overcome the problems encountered with staining
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* Všechny informace vycházejí z publikovaného vědeckého výzkumu
