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14 resultater
A morphometric study on the effects of ethylene treatment in promoting abscission of tobacco flower pedicels.
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The ultrastructural changes observed in ethylene-induced abscission of tobacco flower pedicels (Nicotiana tabacum L. ;Little Turkish') were studied by the techniques of morphometric analysis. The surface area of the membranes, relative volume of the organelles, and the number of organelles were
Fine structure of abscission zones : III. Cytoplasmic changes in abscising pedicels of tobacco and tomato flowers.
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Changes in the cells comprising the abscission zones of Lycopersicon esculentum and Nicotiana tabacum flower pedicels were observed, apparently associated with the separation of the flowers from the plants. Special attention is devoted to changes which occurred in the fine structure of intracellular
Use of the signal peptide of Pisum vicilin to translocate beta-glucuronidase in Nicotiana tabacum.
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A hybrid protein system was used for the study of protein transport in plant cells. A nucleotide sequence (vic) encoding a putative signal peptide of 15 amino acid residues, derived from the published aa sequence of one Pisum vicilin, was synthesized and fused in frame to the gus gene encoding a
Cytology of budding and cleavage in tobacco mesophyll protoplasts cultivated in saline medium.
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Tobacco (Nicotiana tabacum L., cv. Maryland) mesophyll protoplasts cultivated in saline medium divide by bud formation, migration of one nucleus into the bud, and subsequent furrowing. This process was investigated light and electron microscopically. The cytoplasm of the growing bud is richer in
A plasma membrane-anchored fluorescent protein fusion illuminates sieve element plasma membranes in Arabidopsis and tobacco.
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Rapid acquisition of quantitative anatomical data from the sieve tubes of angiosperm phloem has been confounded by their small size, their distance from organ surfaces, and the time-consuming nature of traditional methods, such as transmission electron microscopy. To improve access to these cells,
Development and enzyme activity of protein bodies in proteinoplasts of tobacco root cells.
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The development of protein bodies in proteinoplasts of tobacco (Nicotiana tabacum L. var. Wis. 38) roots was investigated with TEM, HVEM, and enzyme cytochemistry. These plastids contain a three-dimensional network of fenestrated tubules which originate from invaginations of the inner membrane of
Relevant elements of a maize gamma-zein domain involved in protein body biogenesis.
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The N-terminal proline-rich domain of γ-zein (Zera) plays an important role in protein body (PB) formation not only in the original host (maize seeds) but in a broad spectrum of eukaryotic cells. However, the elements within the Zera sequence that are involved in the biogenesis of PBs have not been
Accumulation of 15-Kilodalton Zein in Novel Protein Bodies in Transgenic Tobacco.
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Zeins, the seed storage proteins of maize, are a group of alcohol-soluble polypeptides of different molecular masses that share a similar amino acid composition but vary in their sulfur amino acid composition. They are synthesized on the rough endoplasmic reticulum (ER) in the endosperm and are
Opaque1 encodes a myosin XI motor protein that is required for endoplasmic reticulum motility and protein body formation in maize endosperm.
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Myosins are encoded by multigene families and are involved in many basic biological processes. However, their functions in plants remain poorly understood. Here, we report the functional characterization of maize (Zea mays) opaque1 (o1), which encodes a myosin XI protein. o1 is a classic maize seed
Roles of different forms of lipopolysaccharides in Ralstonia solanacearum pathogenesis.
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Lipopolysaccharides (LPS) are critical components for the fitness of most gram-negative bacteria. Ralstonia solanacearum causes a deadly wilting disease in many crops; however, the pathogenic roles of different forms of LPS and their pathways of biogenesis remain unknown. By screening for
Actin filaments are dispensable for bulk autophagy in plants.
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Actin filament, also known as microfilament, is one of two major cytoskeletal elements in plants and plays important roles in various biological processes. Like in animal cells, actin filaments have been thought to participate in autophagy in plants. However, surprisingly, in this study we found
The lipopolysaccharide of the crop pathogen Xanthomonas translucens pv. translucens: chemical characterization and determination of signaling events in plant cells.
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Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates,
Higher accumulation of F1-V fusion recombinant protein in plants after induction of protein body formation.
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Improving foreign protein accumulation is crucial for enhancing the commercial success of plant-based production systems since product yields have a major influence on process economics. Cereal grain evolved to store large amounts of proteins in tightly organized aggregates. In maize, gamma-Zein is
Nodal endoplasmic reticulum, a specialized form of endoplasmic reticulum found in gravity-sensing root tip columella cells.
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The endoplasmic reticulum (ER) of columella root cap cells has been postulated to play a role in gravity sensing. We have re-examined the ultrastructure of columella cells in tobacco (Nicotiana tabacum) root tips preserved by high-pressure freezing/freeze-substitution techniques to gain more precise
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