Redox Regulation of Satellite Cells and Skeletal Muscle Healing
Λέξεις-κλειδιά
Αφηρημένη
Περιγραφή
A total number of 40-60 males, young individuals aged 18-30 years, will be initially enrolled in the study. Then, participants will be allocated to either a 1) Low Respondents (LR) or a 2) High Respondents (HR) group based on the basal satellite cells content of their vastus lateralis muscle of their dominant leg. In a double-blind, crossover, repeated measures design, participants will consume either Placebo (PLA) or N-acetylcysteine (NAC) before (7-day loading phase), on exercise day and for 8 consecutive days following a single bout of intense exercise (300 eccentric contractions at 30 deg/sec in an isokinetic dynamometer). In both conditions, blood samples and muscle biopsies will be collected at baseline, before the exercise protocol and at 2- and 8-days post-exercise. Muscle performance and soreness will also be assessed at the same time points. Before the first trial, participants' dietary intake (via diet recalls) and physical activity (via accelerometry) will be analyzed and nutritional plans will be provided to participants in order to normalize their antioxidant and protein intake. A 4-week washout period will be implemented between trials. Blood samples will be analyzed for inflammation and oxidative stress markers. Muscle samples will be analyzed for satellite cells responses and myogenic potential, protein levels of intracellular signaling proteins, muscle thiols and antioxidant enzymes activity.
Ημερομηνίες
| Τελευταία επαλήθευση: | 05/31/2020 |
| Πρώτα υποβλήθηκε: | 10/10/2018 |
| Υποβλήθηκε εκτιμώμενη εγγραφή: | 10/15/2018 |
| Δημοσιεύτηκε για πρώτη φορά: | 10/18/2018 |
| Υποβλήθηκε τελευταία ενημέρωση: | 06/16/2020 |
| Δημοσιεύτηκε η τελευταία ενημέρωση: | 06/18/2020 |
| Ημερομηνία έναρξης της πραγματικής μελέτης: | 07/21/2019 |
| Εκτιμώμενη κύρια ημερομηνία ολοκλήρωσης: | 12/31/2020 |
| Εκτιμώμενη ημερομηνία ολοκλήρωσης μελέτης: | 05/04/2022 |
Κατάσταση ή ασθένεια
Παρέμβαση / θεραπεία
Dietary Supplement: N-Acetylcysteine
Dietary Supplement: Placebo
Φάση
Ομάδες βραχιόνων
| Μπράτσο | Παρέμβαση / θεραπεία |
|---|---|
| Experimental: N-Acetylcysteine N-Acetylcysteine supplementation: Orally, 40 mg/kg per day in 3 doses (250 ml each) for 7 consecutive days and immediately post-exercise. The remaining 8 days, 40mg/kg per day in 3 doses (250 ml each). | Dietary Supplement: N-Acetylcysteine N-Acetylcysteine in a powder form diluted in a 250 ml drink containing 248 ml water and 2 ml of natural, non-caloric, flavoring-sweetener containing sucralose. |
| Active Comparator: Placebo Placebo administration: Orally 750 ml per day in 3 doses (250 ml each) for 7 consecutive days and immediately post-exercise. The remaining 8 days, 750 ml per day in 3 doses (250 ml each). | Dietary Supplement: Placebo Placebo consisted of 248 ml water and 2 ml of natural, non-caloric, flavoring-sweetener containing sucralose. |
Κριτήρια καταλληλότητας
| Επιλέξιμες ηλικίες για μελέτη | 18 Years Προς την 18 Years |
| Φύλα επιλέξιμα για μελέτη | Male |
| Δέχεται υγιείς εθελοντές | Ναί |
| Κριτήρια | Inclusion Criteria: 1. No recent history of musculoskeletal injury 2. Non-smokers. 3. Abstain from any vigorous physical activity during the study 4. Abstain from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, NSAIDs and medications before (at least 6 months) and during the study. Exclusion Criteria: 1. A known NAC intolerance or allergy 2. A recent febrile illness 3. A recent history of muscle lesion and/or lower limb trauma 4. Presence of metabolic diseases 5. Use of anti-inflammatory medication. 6. Use of medication interacting with muscle metabolism. |
Αποτέλεσμα
Πρωτεύοντα αποτελέσματα
1. Change in muscle satellite cells number (i.e. Pax7+ cells) and activation status (i.e. Pax7+/MyoD+ cells) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
2. Change in muscle myogenic mRNA expression [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
3. Change in muscle inflammatory state [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
4. Change in intracellular antioxidant enzymes in muscle [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
5. Change in muscle thiol content [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
6. Change in intracellular signaling proteins in muscle [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
7. Change in skeletal muscle damage levels [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
Δευτερεύοντα αποτελέσματα
1. Resting metabolic rate (RMR) [At baseline]
2. Body composition [At baseline]
3. Maximal oxygen consumption (VO2max) [At baseline]
4. Isokinetic strength [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
5. Change in delayed onset of muscle soreness (DOMS) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
6. Physical activity [At baseline.]
7. Dietary intake [At baseline.]
8. Interleukin-1β (IL-1β) in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
9. Interleukin-6 (IL-6) in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
10. Interleukin-8 (IL-8) in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
11. Interleukin-10 (IL-10) in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
12. Cortisol in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
13. Protein carbonyls (PC) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
14. Malondialdehyde (MDA) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
15. Total antioxidant capacity (TAC) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
16. Catalase [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
17. Creatine Kinase (CK) [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
18. White blood cell count in blood [At baseline, before the exercise protocol and at days 2 and 8 following exercise.]
