Identification of an oleosin-like gene in seagrass seeds.
Λέξεις-κλειδιά
Αφηρημένη
OBJECTIVE
To investigate the oil body protein and function in seeds of mature seagrass, Thalassia hemprichii.
RESULTS
Seeds of mature seagrass T. hemprichii when stained with a fluorescent probe BODIPY showed the presence of oil bodies in intracellular cells. Triacylglycerol was the major lipid class in the seeds. Protein extracted from seagrass seeds was subjected to immunological cross-recognition with land plant seed oil body proteins, such as oleosin and caleosin, resulting in no cross-reactivity. An oleosin-like gene was found in seagrass seeds. Next generation sequencing and sequence alignment indicated that the deduced seagrass seed oleosin-like protein has a central hydrophobic domain responsible for their anchoring onto the surface of oil bodies. Phylogenetic analysis showed that the oleosin-like protein was evolutionarily closer to pollen oleosin than to seed oleosins.
CONCLUSIONS
Oil body protein found in seagrass seeds represent a distinct class of land seed oil body proteins.
