Evaluation of Biological Biomarkers Diagnostic of Toxoplasmosis Uveitis

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Abstract

Toxoplasmosis affects one to two newborn each 10000 births. Among them, 1 to 2 % develop learning disabilities or die, and 4 to 27 % develop a chorioretinitis sometimes leading to an amblyopia responsible for visual impairment. Toxoplasmosis uveitis affects too adults immunocompetent and immunodepressed who have had an acquired toxoplasmosis. Clinical diagnosis of ocular toxoplasmosis is more complicated in presence of posterior neuro-retinitis, inflammation of the papilla, uveitis without chorioretinitis, fuchs heterochromic iridocyclitis, scleritis, diffuse necrotizing or multifocal retinitis. In this situation biological markers diagnostic and prognostic of toxoplasmosis uveitis are useful.

Highly kept molecules (during evolution) like stress proteins (Hsp) are are found in the host and the pathogen and there can trigger a crossed immune response. Stress proteins haven't been explored yet, in the context of toxoplasmosis uveitis on humans.

The hypothesis is that Hsp70 and antibodies anti-Hsp70 are diagnostic and prognostic markers of ocular toxoplasmosis.

The goal is to evaluate diagnosis value of biological markers (Hsp70 and antibodies IgG anti-Hsp70) in toxoplasmosis uveitis.

Dates

Last Verified:	06/30/2016
First Submitted:	07/20/2016
Estimated Enrollment Submitted:	07/21/2016
First Posted:	07/24/2016
Last Update Submitted:	07/21/2016
Last Update Posted:	07/24/2016
Actual Study Start Date:	12/31/2009
Estimated Primary Completion Date:	12/31/2015
Estimated Study Completion Date:	12/31/2015

Condition or disease

Subjects Clinically Suspected an Active Source of Toxoplasmosis Chorioretinitis Infection

Intervention/treatment

Other: Subjects suspected of toxoplasmosis chorioretinitis infection

Biological: Subjects suspected of toxoplasmosis chorioretinitis infection

Drug: Subjects suspected of toxoplasmosis chorioretinitis infection

Other: Subjects suspected of toxoplasmosis chorioretinitis infection

Phase

Arm Groups

Arm	Intervention/treatment
Other: Subjects suspected of toxoplasmosis chorioretinitis infection Subjects clinically suspected at least of one active source of toxoplasmosis chorioretinitis infection	Other: Subjects suspected of toxoplasmosis chorioretinitis infection

Eligibility Criteria

Sexes Eligible for Study

All

Accepts Healthy Volunteers

Yes

Criteria

Inclusion Criteria:

- Age over 18 years

- Subjects clinically suspected at least of one active source of toxoplasmosis chorioretinitis infection

- Persons affiliated to national social security

Exclusion Criteria:

- Pregnant, parturient or breastfeeding women

- Persons deprived of liberty by judicial or administrative decision, person under legal protection

- Refusal by a patient to do the PCA (anterior chamber puncture)

- Patients whose following will be difficult or nonexistent

Outcome

Primary Outcome Measures

1. ELISA tests [About an hour]

ELISA (enzyme linked immunosorbent assay) tests. Biological markers evaluation : stress protein Hsp70 and antibodies anti-Hsp70.

Evaluation of Biological Biomarkers Diagnostic of Toxoplasmosis Uveitis

Keywords

toxoplasmosis

inflammation

chorioretinitis

retinitis

scleritis

uveitis

amblyopia