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Garlic With Lime Juice and Chlorhexidine Mouthwash

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Status
Sponsors
Cairo University

Keywords

Abstract

the study about herbal mouthwash. it is clinical trial .the investigator will compare between chlorhexidine mouthwash and garlic with lime juice mouthwash.and their effect on gingival bleeding and plaque and salivary bacteria.

Description

the study about herbal mouth wash. it is clinical trial .the investigator will compare between chlorhexidine mouthwash and garlic with lime juice mouthwash.and show their effect on gingival bleeding and strepococcus bacteria present in saliva and plaque.

1. Preparation of garlic with lime juice will occur under aseptic condition.and give it to the first group.

provide chlorhexidine to the second group.

2. Collection of plaque and saliva samples will be obtained from children who fulfilled the inclusion criteria.

3. Asses gingival condition by asking patient.

4. Subjects will be instructed to rinse for 14 days, twice daily.

5. Morning after breakfast and night before going to bed.

6. With 10 ml (undiluted) of the assigned mouthwash for 30 s and then expectorate the rinse.

7. Again after 14 days, the saliva and plaque samples will be taken and inoculated on blood agar plates to determine the colony count and the gingival index by loe and illness will be assessed.

Dates

Last Verified: 03/31/2017
First Submitted: 03/20/2017
Estimated Enrollment Submitted: 04/03/2017
First Posted: 04/09/2017
Last Update Submitted: 04/03/2017
Last Update Posted: 04/09/2017
Actual Study Start Date: 03/09/2017
Estimated Primary Completion Date: 05/31/2017
Estimated Study Completion Date: 05/31/2017

Condition or disease

Gingival Bleeding

Intervention/treatment

Drug: garlic with lime juice mouthwash

Drug: chlorhexidine mouthwash

Phase

-

Arm Groups

ArmIntervention/treatment
Experimental: garlic with lime juice mouthwash
garlic with lime juice mouthwash is herbal antimicrobial .children were instructed to rinse for 14 days, twice daily, ,10 ml (undiluted) for 30 second and then expectorate . To prepare garlic with lime mouth rinse, 100 g of fresh, washed garlic cloves was macerated in a sterile, ceramic mortar and water was added to obtain a homogenate which was then filtered off with a sterile muslin cloth. the final concentration of the solution was determined to be 1 g/100 ml. About 100 ml of lime juice was extracted from fresh lemons using a juice extractor and added to the garlic extract.
Drug: garlic with lime juice mouthwash
garlic with lime juice is herbal antimicrobial 10 ml (undiluted) for 30s and then expectorate for 14 days.o prepare garlic with lime mouth rinse, 100 g of fresh, washed garlic cloves was macerated in a sterile, ceramic mortar and water was added to obtain a homogenate which was then filtered off with a sterile muslin cloth. the final concentration of the solution was determined to be 1 g/100 ml. About 100 ml of lime juice was extracted from fresh lemons using a juice extractor and added to the garlic extract.
Active Comparator: chlorhexidine mouthwash
chlorhexidine is antimicrobial, antiplaque mouthwash 10 ml (undiluted) for 30 second twice daily and then expectorate for 14 days. it is commercial mouthwash .
Drug: chlorhexidine mouthwash
hlorhexidine is antimicrobial, antiplaque mouthwash 10 ml (undiluted) for 30 second twice daily and then expectorate for 14 days.

Eligibility Criteria

Ages Eligible for Study 9 Years To 9 Years
Sexes Eligible for StudyAll
Accepts Healthy VolunteersYes
Criteria

Inclusion criteria:

1. Children aged between 9 and 12 years.

2. Children are having normal occlusion (no crowding), non-compromised oral health (brushed their teeth once-daily) using toothbrush and non-fluoridated toothpaste. And practicing no other oral hygiene measures.

3. Medically free history.

4. Absence of orthodontic appliances.

Exclusion criteria:

1. History of current or recent (at least for the past 1 month) antibiotic usage or other medications.

2. Presence of Abscess, draining sinus, cellulitis, or other conditions requiring emergency dental treatment.

3. not willing to comply with the study.

Outcome

Primary Outcome Measures

1. gingival bleading [15 day]

bleeding of gingiva asses by yes or no

Secondary Outcome Measures

1. Streptococcal count in saliva and plaque [15 day]

Streptococcal count in saliva and plaque

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