Investigative Ophthalmology and Visual Science 1987-Mar

Delta 1-pyrroline-5-carboxylate dehydrogenase in the bovine ciliary body and iris.

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T Shiono

S Hayasaka

K Mizuno

ARTICLE: 3557858

BioSeek: 3557858

Keywords

Abstract

Delta 1-pyrroline-5-carboxylate dehydrogenase extracted from the combined ciliary body and iris of bovine eyes was studied biochemically. The enzyme was purified 120-fold. The partially purified enzyme had broad optimum at pH 8.0. Apparent Km values for DL-delta 1-pyrroline-5-carboxylate and NAD were 0.14 and 0.18 mM, respectively. The enzyme activity was strongly inhibited by GABA, proline, hydroxyproline, and glutamine.

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