[Study on the preparation and quality standard of Ephedra sinica dispensing granules].
Keywords
Abstract
OBJECTIVE
To optimize the preparation process and to set up the quality standard of Ephedra sinica Dispensing Granules.
METHODS
Nine experiments were carried out by L9 (3(4)) orthogonal design. Ephedra sinica Dispensing Granules was identified by TLC and the content of ephedrine was determined by HPLC.
RESULTS
The optimal extraction process was as follows: the water that 10 times of Ephedra sinica, was decocted 3 times and each time was sustained for 1.5 hours. The linear relationship of ephedrine hydrochloride was at the range of 0.0081 - 0.1377 microg (r = 0.999 9). The average recovery was 100.25% ( RSD = 2.24%, n = 6).
CONCLUSIONS
The extraction process is scientific. The method is available with a good reproducibility and can control the quality of Ephedra sinica Dispensing Granules.
