Serum albumin was assayed with bromcresol purple using routine clinical chemical photometric analysers. Bromcresol purple and bromcresol green dyes were compared, and albumin assays using these dyes were compared to those made by radial immunodiffusion and rocket immunoelectrophoresis. Bromcresol
Correct reference intervals are an important part of test results. As establishing own reference intervals is a very expensive task, the NORIP reference intervals are often transferred for use in Nordic laboratories. The NORIP reference interval on P-Albumin was here compared to current results for
The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon
BACKGROUND
In clinical settings, human serum albumin (HSA) is widely quantitated on the basis of change in the color of bromocresol purple (BCP) bound to HSA. However, the binding sites of BCP on HSA are hitherto unknown. We have identified these sites by performing binding experiments in which BCP
The bromocresol purple methods for the determination of serum albumin on the Du Pont aca and on the KONE CD show very acceptable linear ranges and imprecision data. Comparison of methods showed good agreement between albumin determinations on the two instruments. Human based quality control
BACKGROUND
Albumin measured by a bromcresol purple dye-binding assay (Alb(BCP)) agrees more closely with the gold standard of immunonephelometry than does bromcresol green (Alb(BCG)) measurement. Both tests are in current clinical use. A method for converting between the two would be
We describe a new automated dye-binding method for serum albumin determination with bromcresol purple (BCP) that has several advantages over an existing bromcresol green (BCG) method. The continuous-flow method is sensitive, linear, and precise, with negligible sample interaction at an analytical
Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic
The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for
The interaction of bromocresol purple (BCP) anions with bovine serum albumin (BSA) was investigated by principal factor analysis method, and reaction model, the number of molecular species and spectra of each component present in the reaction mixture were determined. The number of molecular species
We used automated and manual versions of two commonly used dye-binding methods for albumin to quantify albumin that had been modified by covalent addition of bilirubin. Dye-binding methods based on reaction with bromcresol purple underestimated the modified albumin by as much as 29%. Dye-binding
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