Natural Antioxidant Ice-cream, Oxidative Stress and Vascular Function
Palabras clave
Abstracto
Descripción
14 healthy, non-smoking, volunteers (7 males ), aged between 20 and 40 years were enrolled. The study was randomized, placebo-controlled. After enrollment, 3 days before entering the study, all subjects performed an exercise stress test. They were then randomly allocated to the treatment sequence with 100 mg of natural anti-oxidant ice cream or milk chocolate ice-cream (control icecream) in a cross-over single-blind design. There was 1 week wash-out between 2 phases of the study.
Markers of oxidative stress (D-ROMs and H2O2); serum antioxidant status (FRAP), NO bioavailability (NOx), the endothelium flow-mediated vasodilation (FMD and the RHI by Endopat 2000) were evaluated in fasting condition at baseline (time 0) and 2 hours after the ingestion of the anti-oxidant or control ice-creams. The exercise stress test was performed before randomization and 2 hours after each ice-cream meal test. In both experiments, the ice-cream was consumed within 10 min.
Each subject was asked to refrain from any kind of medication, diet integrators, intensive physical activity and to avoid consumption of polyphenols rich foods, such cocoa-containing products, red wine, tea within the previously 2 weeks. All participants gave written informed consent, and the study was approved by the Medical Ethics Committee of La Sapienza University of Rome (Rif: 3727, Prot: 2325/15).
fechas
Verificado por última vez: | 01/31/2016 |
Primero enviado: | 02/07/2016 |
Inscripción estimada enviada: | 02/11/2016 |
Publicado por primera vez: | 02/17/2016 |
Última actualización enviada: | 02/17/2016 |
Última actualización publicada: | 02/18/2016 |
Fecha de inicio real del estudio: | 06/30/2015 |
Fecha estimada de finalización primaria: | 09/30/2015 |
Fecha estimada de finalización del estudio: | 11/30/2015 |
Condición o enfermedad
Intervención / tratamiento
Other: Antioxidant Ice-cream
Other: Control Ice-cream
Fase
Grupos de brazos
Brazo | Intervención / tratamiento |
---|---|
Experimental: Antioxidant Ice-cream Natural antioxidant Ice-cream | Other: Antioxidant Ice-cream Narutal Antioxidant ice-cream |
Placebo Comparator: Control Ice-cream Natural control ice-cream | Other: Control Ice-cream Natural Control Ice-cream |
Criterio de elegibilidad
Edades elegibles para estudiar | 20 Years A 20 Years |
Sexos elegibles para estudiar | All |
Acepta voluntarios saludables | si |
Criterios | Inclusion Criteria: - healthy, non-smoking, volunteers Exclusion Criteria: - Assumption of any kind of medication, diet integrators, intensive physical activity and to avoid consumption of polyphenols rich foods, tea within the previously 2 weeks. |
Salir
Medidas de resultado primarias
1. Endothelial function [Acute 2 hours]