A new rat model of acute seizures induced by tutin.

Coriaria Lactone (CL) is a mixture that has been used to establish animal models of epilepsy. In this study, we focused on the epileptogenic action of tutin, a pure chemical component derived from CL. Rats were implanted with a guide cannula for microinjection of tutin into either of the lateral cerebral ventricles. Behavioral and electroencephalographic (EEG) changes were investigated for at least 2 h after tutin administration. Injected animals presented behavioral seizures: initially, facial and limbic clonus, and subsequently, tonic-clonic seizures that eventually progressed to status epilepticus. Accompanying the behavioral activities, a variety of EEG patterns were recorded. Spike-and-wave complexes occurred continuously at 3 Hz, with a mean amplitude of approximately 295 microV. Multiple spikes and slow waves occurred repetitively and became more frequent and intense. The amplitude of this EEG pattern was low (approximately 85 microV) at onset and gradually increased to approximately 200 microV. Spikes (8 Hz, approximately 555 microV) and slow waves (3 Hz, approximately 670 microV) occurred periodically at the onset of grand mal seizures. Behavioral and EEG changes induced in rats by tutin demonstrated that this is a potent convulsant, by which a new animal model of status epilepticus was established. This acute seizure model is productive and would be optional for investigation of seizures or status epilepticus.