Clinical and Vaccine Immunology 2010-Jan
Elimination of false-positive results in a luminex assay for pneumococcal antibodies.
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Abstracto
In our 14-valent Luminex assay for pneumococcal antibodies, we identified two groups of sera that caused false-positive results. The first group bound nonspecifically to the Luminex microspheres. The second group reacted specifically with bovine serum albumin (BSA). We describe here methods that eliminated the false-positive reactivity of both groups.