Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies

In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet by vacuum filtration technique using Buckner funnel. Phase crystallization, fluorescence property and microstructure of eHAp were confirmed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Photoluminiscence spectroscopy (PL) and Field emission scanning electron microscopy (FESEM). Strong antimicrobial activity was observed for EU, TTO and WO on both E. coli and S. aureus mediated by cell membrane damage and leakage of cytoplasmic components. The oil absorbed eHAp nanocomposites were found to be moderately biocompatible with normal WI-38 cells up to MIC concentration various time scale. The nanocomposites showed significant cytotoxic activity on breast cancer cell line MDA-MB 468 and the fluorescent property of the eHAp was utilized to visualize internalization of particles in the cells. The release profile of the oils from the eHAp matrix showed pH dependent release indicated that the porous matrix can be used as a suitable carrier for modulated and sustained release of bioactive components. Thus, given the multifunctional attributes these natural essential oil-based nanocomposites show great promise as an alternative to conventional therapeutic treatments.

Keywords: Anticancer; Cytotoxicity; Essential oil; Fluorescence; Hydroxyapatite.