Water decoctions from the leaves of Taxus cuspidata are used in traditional Chinese medicine to treat cancer, suggesting that water soluble constituents from these leaves may possess anticancer properties. Interestingly, hydrophilic paclitaxel derivatives, as opposed to paclitaxel itself, can be
A new separation method, negative pressure cavitation (NPC) extraction followed by HPLC-MS-MS for the determination of paclitaxel and its analogues in the needles of Taxus species is described in this study. Compared with three conventional extraction methods, NPC is a more effective, economical,