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acetyldigoxin/digitalis lanata
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7 resultados
High-performance liquid chromatographic determination of alpha-acetyldigoxin in Digitalis lanata leaves.
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An analytical method for the determination of alpha-acetyldigoxin in Digitalis lanata leaves by HPLC has been developed. The procedure consists of extraction of dry leaf powder with 50% methanol and cleanup by a Sep-Pak C18 cartridge prior to HPLC analysis. The quantitation is carried out by the
Extraction and quantitation of digoxin and acetyldigoxin from the Digitalis lanata leaf via near-supercritical methanol-modified carbon dioxide.
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An extraction process is reported that employs a near-supercritical mixture of CO2 and MeOH to extract the cardiac glycoside, digoxin, from the Digitalis lanata leaf. The method development of the sample preparation procedure is presented in detail, and reasons for trends that occur in the natural
Acetyldigoxin and acetyldigitoxin from digitalis lanata.
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UDP-glucose:digitoxin 16'-O-glucosyltransferase from suspension-cultured Digitalis lanata cells.
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Suspension cultures from several cell lines of Digitalis lanata, as well as cultures from 6 other plant species were checked for their ability to form purpurea-glycoside A from digitoxin. An in-vitro assay for the UDP-glucose:digitoxin 16'-O-glucosyltransferase (DGT, EC 2.4.1.-) has been established
Cardenolide and Neutral Lipid Biosynthesis from Malonate in Digitalis lanata.
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DIGITALIS LANATA plants were grown on water culture in a controlled environment and in the young, growing leaves free sterols (0.335 micromol/g FW), triacylglycerols (0.97 micromol/g FW) and cardenolides (1.82 micromol/g FW) were the major apolar and polar lipids. The cardenolide-containing fraction
High-resolution tandem mass spectrometry dataset reveals fragmentation patterns of cardiac glycosides in leaves of the foxglove plants.
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Cardiac glycosides, steroid derivatives extracted from the foxglove plants, have been used for the treatment of heart failure since the 18th century. A method based on liquid chromatography coupled with high-resolution tandem mass spectrometry (LC/MS2) has been developed to characterize
Profiling and structural analysis of cardenolides in two species of Digitalis using liquid chromatography coupled with high-resolution mass spectrometry.
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Plants of the Digitalis genus contain a cocktail of cardenolides commonly prescribed to treat heart failure. Cardenolides in Digitalis extracts have been conventionally quantified by high-performance liquid chromatography yet the lack of structural information compounded with possible co-eluents
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