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Mouse lethal activity of a HEp-2 vacuolation factor, cereulide, produced by Bacillus cereus isolated from vomiting-type food poisoning.

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The HEp-2 vacuolation factor (or cereulide) produced by Bacillus cereus isolated from vomiting-type food poisoning, which is supposed to induce emesis, was found to give mouse and suncus lethality after intravenous and intraperitoneal administration. The emetic activity of the factor was also found

Production of the vacuolation factor of Bacillus cereus isolated from vomiting-type food poisoning.

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Vacuole response in HEp-2 cells was induced with culture supernatants of Bacillus cereus strains isolated from outbreaks of vomiting- and diarrheal-type food poisoning grown in rice flour and laboratory media. High vacuole response was obtained with culture supernatants of B. cereus strains isolated

Properties and production characteristics of vomiting, diarrheal, and necrotizing toxins of Bacillus cereus.

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Evidence is provided that the enterotoxin of Bacillus cereus variously described in the literature as diarrheagenic toxin, diarrheal agent, fluid accumulation factor, vascular permeability factor, dermonecrotic toxin, and intestinonecrotic toxin is a single relatively unstable protein of molecular

Emesis of rhesus monkeys induced by intragastric administration with the HEp-2 vacuolation factor (cereulide) produced by Bacillus cereus.

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To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting-type food poisoning, some properties and emetic activities of both purified HEp-2 factor (cereulide) and partially purified factor to rhesus monkeys were

Identification of emesis-causing Bacillus cereus strains by polymerase chain reaction: preliminary results.

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[Bacillus cereus septicemia in a patient with severe aplastic anemia].

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A 78-year-old female was admitted with complaints of malaise and fatigue in the legs. The patient was diagnosed as severe aplastic anemia and treatment was started with metenolone and steroid pulse therapy. Administration of antibiotics and granulocyte-colony stimulating factor which led to a

[Bacillus cereus enterotoxins].

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Data about Bacillus cereus different enterotoxins including hemolysin (HBL), nonhemolytic enterotoxin (NHE), enterotoxin (T), and emesis-inducing thermostable enterotoxin (ETE) are summarized in the article. Information about synthesis of different diarrhea-inducing and emesis-inducing enterotoxins,

Emetic disease caused by Bacillus cereus after consumption of tuna fish in a beach club.

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Several vomiting episodes were reported in December 2007 by the management of a beach club in Calvià. The subsequent case investigation confirmed tuna fish consumption a few hours before onset of emesis in all cases. Microbiological analyses detected high bacterial levels in ready-to-eat fish

[Epidemic outbreak in a home for the aged caused probably by Bacillus cereus].

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OBJECTIVE To identify the causative agent and the factors precipitating the outbreak. METHODS Observational, crossover study. METHODS Las Delicias Health district, Jerez de la Frontera. METHODS The population exposed, belonging to an elderly persons' home. RESULTS The total number of people exposed

Inhibition of human natural killer cell activity by cereulide, an emetic toxin from Bacillus cereus.

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The lipophilic toxin, cereulide, emitted by emetic food poisoning causing strains of Bacillus cereus, is a powerful mitochondria toxin. It is highly lipophilic and rapidly absorbed from the gut into the bloodstream. We tested how this toxin influences natural killer (NK) cells, which are important

[The importance of Bacillus cereus in food poisoning].

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In the light of the available literature the present knowledge on the role of Bacillus cereus in alimentary intoxications is discussed, particularly the occurrence and role of B. cereus in food pollution, the most frequent alimentary intoxications in the world are reviewed, the biological tests on

One-day pulsed-field gel electrophoresis protocol for rapid determination of emetic Bacillus cereus isolates.

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Bacillus cereus, the Gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. Rapid determination of cereulide-positive B. cereus isolates is of highest importance due to consequences of this intoxication

Characterization of three Bacillus cereus strains involved in a major outbreak of food poisoning after consumption of fermented black beans (Douchi) in Yunan, China.

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Three Bacillus cereus strains isolated from an outbreak of food poisoning caused by the consumption of fermented black beans (douchi) containing B. cereus is described. The outbreak involved 139 persons who had nausea, vomiting, and diarrhea. The strains were isolated from vomit and the unprepared

Novel Sequence Type in Bacillus cereus Strains Associated with Nosocomial Infections and Bacteremia, Japan.

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Bacillus cereus is associated with foodborne illnesses characterized by vomiting and diarrhea. Although some B. cereus strains that cause severe extraintestinal infections and nosocomial infections are recognized as serious public health threats in healthcare settings, the genetic backgrounds of B.

Serotypes of Bacillus cereus from outbreaks of food poisoning and from routine foods.

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A provisional serotyping scheme was used to type cultures of Bacillus cereus from 84 outbreaks of food poisoning in seven countries; 283 of the 337 (84%) cultures tested were typable. In 35 of the 61 outbreaks associated with a vomiting-type syndrome, foods, clinical specimens or both yielded

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