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[False negative diagnosis of HIV-1].
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We report a case of a false negative diagnosis of HIV-1 infection in an African girl. Two HIV-1 DNA polymerase chain reaction (PCR) tests were negative at the second and fourth months of life. Because anti-HIV antibodies persisted when the patient was 18 months old, the HIV-1 RNA PCR test was
False positive prenatal diagnosis of cystic fibrosis by protease assay.
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False-negative results with methylumbelliferylguanidinobenzoate reactive proteases in cystic fibrosis pregnancies.
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Use of high-throughput mass spectrometry to reduce false positives in protease uHTS screens.
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As a label-free technology, mass spectrometry (MS) enables assays to be generated that monitor the conversion of substrates with native sequences to products without the requirement for substrate modifications or indirect detection methods. Although traditional liquid chromatography (LC)-MS methods
Using proteases to avoid false identification of DNA-protein complexes in gel shift assays.
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Gel mobility shift assays using crude nuclear extracts may result in the formation of multiple DNA-protein complexes reflected by their discrete gel mobilities. Identification of the multiple complexes can sometimes be complicated by the presence of protease activities in the extract as demonstrated
Development of peptide receptor binding assays: methods to avoid false negatives.
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Selection of appropriate ligand receptor binding assay conditions is critical for peptides, where the possibility of obtaining false negative results is pertinent due to their inherent adsorption and instability characteristics, as well as high response-sensitivity to operational conditions. The aim
Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.
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Rabbits and guinea pigs were immunized with purified bovine rotavirus. Immunoglobulin G fractions of the resulting antisera were used in a standard four-layer solid-phase enzyme immunoassay (EIA) for rotavirus antigen in human faecal specimens. Samples negative for rotavirus in electron microscopy,
Distinguishing binders from false positives by free energy calculations: fragment screening against the flap site of HIV protease.
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Molecular docking is a powerful tool used in drug discovery and structural biology for predicting the structures of ligand-receptor complexes. However, the accuracy of docking calculations can be limited by factors such as the neglect of protein reorganization in the scoring function; as a result,
Using skimmed milk agar to functionally screen a gut metagenomic library for proteases may lead to false positives.
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OBJECTIVE
Functional screens using skimmed-milk agar to obtain protease activity is a common approach. The aim of this study was to determine the efficacy of this screen to obtain protease activity from a metagenomic library.
RESULTS
A distal gut metagenomic library was functionally screened using a
Use of parallel validation high-throughput screens to reduce false positives and identify novel dengue NS2B-NS3 protease inhibitors.
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Dengue virus (DENV), a mosquito-borne member of the family Flaviviridae, is a significant global pathogen affecting primarily tropical and subtropical regions of the world and placing tremendous burden on the limited medical infrastructure that exists in many of the developing countries located
Stool desorbing activity: a possible cause of false-positive reactions in competitive enzyme immunoassays.
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We have developed a competitive enzyme immunoassay for the measurement of purified toxin A of Clostridium difficile. However, when we applied this assay to the detection of C. difficile toxin in stool specimens, we noted a high rate of nonspecific activity in fecal specimens which did not contain
Prenatal diagnosis of cystic fibrosis: false negative result with the 4-methylumbelliferyl-p-guanidinobenzoate assay for proteases in amniotic fluid.
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Failure to detect type-C virus p30-related antigen in systemic lupus erythematosus: false-positive reaction due to protease activity.
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Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false-positive control.
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Proteolytic treatment of intact bacterial cells is an ideal means for identifying surface-exposed peptide epitopes and has potential for the discovery of novel vaccine targets. Cell stability during such treatment, however, may become compromised and result in the release of intracellular proteins
A printable hydrogel microarray for drug screening avoids false positives associated with promiscuous aggregating inhibitors.
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A significant problem in high-throughput drug screening is the disproportionate number of false hits associated with drug candidates that form colloidal aggregates. Such molecules, referred to as promiscuous inhibitors, nonspecifically inhibit multiple enzymes and are thus not useful as potential
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