A colorimetric, particularly spectrophotometric, method and compositions for determining calcium levels in blood serum in the presence of magnesium and other serum ions is described. The method uses an aqueous composition with a pH between about 7.5 and 10 with arsenazo III to complex calcium and a hydroxyquinoline selected from the group consisting of 8-hydroxyquinoline and 8-hydroxyquinoline-5-sulfonate to complex magnesium. Lipase and a cyclodextrin can be used as reagents to clear triglycerides in the serum which can provide turbidity and false results in some instances. The method is particularly useful for human serum.

The Board of Governors of Wayne State University

Method and compositions for the determination of serum calcium using aersenazo III

A technique and an apparatus are disclosed for the detection of cancer using nuclear magnetic resonance (NMR). Specifically, NMR parameters for protons of lipid methyl and/or methylene groups are determined and compared against a corresponding value for healthy patients. Suppression of the water proton signal is employed where necessary in order to obtain a suitable spectrum for the non-water component protons. In the event that a positive reading is obtained, the level of plasma triglycerides is determined and if it is high, the patient's bodily fluid sample is further subjected to second proton nuclear magnetic spectroscopy. The area or the intensity of the portion correlating to 2.0 and 2.8 ppm of the resonance line generated in the second NMR is measured which discriminates between true and false positive results from the proton NMR reading and determines the presence or absence of cancer in the patient.

The Beth Israel Hospital Association

Method of detecting cancer by measuring lipid-peroxidation using NMR

A technique and an apparatus are disclosed for the detection of cancer using nuclear magnetic resonance (NMR). Specifically, NMR parameters for protons of lipid methyl and/or methylene groups are determined and compared against a corresponding value for healthy patients. In the preferred embodiments, an improved NMR spectrometer is employed to provide a spectrum for non-water components of blood, blood serum or blood plasma and the width of the methyl and/or methylene groups is automatically measured at half-height as a determination of spin-spin relaxation time (T.sub.2) which is the parameter used for purposes of comparison with healthy controls. Suppression of the water proton signal is employed where necessary in order to obtain a suitable spectrum for the non-water component protons. In the event that a positive reading is obtained, the level of plasma triglycerides is determined and if it is high, the patient's bodily fluid sample is further subjected to C-13 nuclear magnetic spectroscopy. The resonance line of the plasma C-13 spectra discriminates between true and false positive results from the proton NMR reading and determines the presence or absence of cancer in the patient.

false/triglyceride

Method and compositions for the determination of serum calcium using aersenazo III

Method of detecting cancer by measuring lipid-peroxidation using NMR

Apparatus and method for detecting cancer using nuclear magnetic resonance

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