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Cytotoxicity of purified cassava linamarin to a selected cancer cell lines.
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Cassava (Manihot esculenta Crantz) is a known source of linamarin, but difficulties associated with its isolation have prevented it from being exploited as a major source. A batch adsorption process using activated carbon proved successful in its isolation, with ultrafiltration playing a pivotal
Autophagy induction as an efficient strategy to eradicate tumors.
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The understanding of the mechanisms of cell-death execution and the role that they play in different diseases opens new therapeutic strategies. Currently, increasing evidence indicates that autophagy is a frequent cell-death mechanism, so the question arises: Could autophagy stimulation be
Therapy mediated by mitophagy abrogates tumor progression.
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Autophagy is mainly a cellular recycling process that promotes survival, but it can also cause cell death if cell injury persists. The role of mitophagy in tumorigenesis remains uncertain. Other cell death types, such as apoptosis or necrosis, are often altered during tumor development and therefore
The adenovirus-mediated linamarase/linamarin suicide system: a potential strategy for the treatment of hepatocellular carcinoma.
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Suicide gene therapy, also known as gene-directed enzyme prodrug therapy, has the potential to provide a cure for hepatocellular carcinoma (HCC), which is a leading cause of cancer death worldwide. Linamarase from cassava is an enzyme that can hydrolyze the innocuous substrate linamarin into
An efficient and high-yielding method for extraction and purification of linamarin from Cassava; in vitro biological evaluation.
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During the last three decades, studies of linamarin extracted from cassava have received increased attention due to the presence of high cyanogenic compounds in these extracts. The methods that are utilized to isolate linamarin are either tedious or use acidic conditions resulting in poor yields. In
Antibody-guided enzyme therapy of cancer producing cyanide results in necrosis of targeted cells.
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A number of enzyme/prodrug activation approaches for the treatment of cancer have been reported to date with varying success. We describe progress in the development of a system based on a beta-glucosidase enzyme in combination with a naturally occurring "prodrug," the sugar linamarin, which
Antiproliferative, antileishmanial and antimicrobial studies on Cnidoscolus quercifolius Pohl (Euphorbiaceae).
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Cnidoscolus quercifolius is an Euphorbiaceae endemic to the northeast region of Brazil, which is used in folk medicine as anti-inflammatory, analgesic and antibiotic. An ethanolic extract was prepared with the leaves from C. quercifolius, and also partitioned and chromatographed
Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles.
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The straightforward production and dose-controlled administration of protein therapeutics remain major challenges for the biopharmaceutical manufacturing and gene therapy communities. Transgenes linked to HIV-1-derived vpr and pol-based protease cleavage (PC) sequences were co-produced as chimeric
Theoretical Analysis for the Safe Form and Dosage of Amygdalin Product.
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