The present invention relates to a uridine diphosphate (UDP)-glycosyltransferase protein which has glycosylation activity for a hydroxyl group at the C-20 position of a protopanaxadiol (PPD)- or protopanaxatriol (PPT)-type ginsenoside, and a method for glycosylation of UDP using the same.

KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

INTELLIGENT SYNTHETIC BIOLOGY CENTER

Korea Advanced Institute of Science and Technology

Intelligent Synthetic Biology Center

Method for glycosylation of ginsenoside using a glycosyltransferase derived from panax ginseng

According to this invention, Prevotella sp. S-1 strain (Bacteroidaceae family, KFCC-10923) is cultured in a medium supplemented with Panax ginseng saponin so as to collect the metabolites of saponin generated and accumulated in the medium, thus ensuring their selective production with high efficiency.This invention related to a process for the preparation of the metabolites of protopanaxadiol saponin, wherein Prevotella sp. S-1 strain (Bacteroidaceae family, KFCC-10923), is cultured at a medium supplemented with Panax ginseng saponin and then, the metabolites of protopanaxadiol saponin contained in the medium--20-O-.beta.-D-glucopyranosyl-20(s)-protopanaxadiol, 20-O-[.alpha.-L-arabinopyranosyl-20(s)-protopanaxadiol, and 20-0-[.alpha.-L-arabinopuranosyl(1.fwdarw.6)-.beta.-D-glucopyranosyl]-20(s )-protopanaxadiol --are generated and accumulated for collecting them thereof.

Il Hwa Co., Ltd.

Happy World Inc.

Process for the preparation of metabolites of Ginseng saponins

The present invention relates to a novel uridine diphosphate (UDP)-glycosyltransferase, and particularly to a novel UDP-glycosyltransferase derived from ginseng and use thereof, a method for preparing glycosylated ginsenoside by converting protopanaxadiol (PPD)-type ginsenoside using the UDP-glycosyltransferase, a composition for converting the PPD-type ginsenoside into glycosylated ginsenoside, comprising the UDP-glycosyltransferase, a transformant or a culture thereof as active ingredients, a method for enhancing the expression of the UDP-glycosyltransferase using MeJA (methyl jasmonate), and a composition for enhancing the expression of the UDP-glycosyltransferase, which comprises MeJA as an active ingredient.

UDP-glycosyltransferase derived from ginseng and use thereof

Disclosed herein is nanoemulsion prepared by emulsifying main metabolites of ginseng saponin obtained by conversion of glucose, i.e. compound K (20-O-.beta.-D-glucopyranosyl-20(S)-protopanaxadiol), ginsenoside F1 (20-O-.beta.-D-glucopyranosyl-20(S)-protopanaxatriol) and compound Y (20-O-[.alpha.-L-arabinopyranosyl(1.fwdarw.6)-.beta.-D-glucopyranosyl]-20- (S)-protopanaxadiol); and admixture thereof, in fine emulsion or liposome with dermotropic emulsifier by nano-emulsification; and having enhanced skin penetration, so to be effective in promoting proliferation of fibroblast and biosynthesis of collagen.

Amorepacific Corporation

Nanoemulsion comprising metabolites of ginseng saponin as an active component and a method for preparing the same, and a skin-care composition for anti-aging containing the same

protopanaxadiol/panax

Method for glycosylation of ginsenoside using a glycosyltransferase derived from panax ginseng

Process for the preparation of metabolites of Ginseng saponins

UDP-glycosyltransferase derived from ginseng and use thereof

Nanoemulsion comprising metabolites of ginseng saponin as an active component and a method for preparing the same, and a skin-care composition for anti-aging containing the same

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