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schisandrol/schisandra chinensis
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Isolation and purification of schisandrol A from the stems of Schisandra chinensis and cytotoxicity against human hepatocarcinoma cell lines.
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BACKGROUND
Schisandrol A, a lignan with anticancer effects, is one of the representative components that identifies Schisandra chinensis.
OBJECTIVE
A method for purifying schisandrol A from the stems of S. chinensis was established using an octadecylsilyl (ODS) column combined with preparative
Schisandrol A from Schisandra chinensis reverses P-glycoprotein-mediated multidrug resistance by affecting Pgp-substrate complexes.
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Recent studies have shown that dibenzocyclooctadiene lignans may reverse P-glycoprotein-mediated multidrug resistance (Pgp-MDR) in cancer cells; however, the mechanism of action remains unknown. Through screening of herbs, we found that schisandrol A (SCH) isolated from Fructus Schisandrae (the
[Chemical constituents from supercritical CO2 extraction of Schisandra chinensis].
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OBJECTIVE
To study the chemical constituents from the supercritical CO2 extraction of Schisandra chinensis.
METHODS
The compounds were separated and purified by conventional column chromatography and their structures were identified by spectroscopic methods.
RESULTS
Nine compounds were isolated from
Lignans in Schisandra chinensis in vitro cultures.
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Contents of schisandrol A and schisandrol B were determined in methanolic extracts of biomass from in vitro cultures of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) using an HPLC method. The biomass was cultured on six variants of Murashige and Skoog (MS) medium containing different
Evaluation of the inhibition of human carboxylesterases (CESs) by the active ingredients from Schisandra chinensis.
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1. Schisandra chinensis, also called wuweizi in Chinese, is the fruit of Schisandra chinensis (Turcz.) Baill., and has been officially utilized as an astringent tonic for more than two thousand years in China. This study aims to evaluate the inhibition of carboxylesterases (CESs) by the major
Lignans with inhibitory activity against NFAT transcription from Schisandra chinensis.
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Seven lignans from Schisandra chinensis were investigated for their inhibitory activity on NFAT transcription. Both gomisin N (IC 50 : 1.33 +/- 0.05 microM) and schisandrol A (IC 50 : 1.34 +/- 0.05 microM) showed higher activity than gomisin E (IC 50 : 4.73 +/- 0.09 microM), schisandrin A (IC 50 :
Purification of lignans from Schisandra chinensis fruit by using column fractionation and supercritical antisolvent precipitation.
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This study examined the use of ultrasonic-assisted extraction (UAE) coupled with column chromatography (CC) and supercritical antisolvent (SAS) precipitation in purifying five lignans from the dried fruit of Schisandra chinensis. Column fractionation of the ultrasonic extracts and SAS precipitation
Rapid analysis of nine lignans in Schisandra chinensis by supercritical fluid chromatography using diode array and mass spectrometric detection.
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Lignans are the bioactive constituents in Schisandra chinensis fruits. For the first time major representatives could directly be determined in plant extracts by using Supercritical Fluid Chromatography. Based on nine commercially available standards the method was developed, finally permitting
Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system.
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An online supercritical fluid extraction with supercritical fluid chromatography system could provide sequential extraction and quantitative analysis of lignans in Schisandra chinensis. Supercritical fluid extraction conditions were optimized at 15 MPa, 50°C, and 4 min with supercritical
[Effect of forchlorfenuron on fruit morphology and lignans content of Schisandra chinensis].
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The effect of plant growth regulator forchlorfenuron (CPPU) 1 x 10(-6), 0.67 x 10(-6), 0.5 x 10(-6) on fruit morphology and effective components lignans was studied. Those morphologies were the combination of four basic morphological changes. The result showed, diametre were increased and
[Intestinal absorption of the effective components of Schisandra chinensis Baill by rats single-pass perfusion in situ].
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The aim of the study is to investigate rat intestinal absorption behavior of three main active components, schisandrol A, schisandrin A and schisandrin B in Schisandra chinensis Baill extracts in intestine of rats. With phenol red as the indicator, in situ single pass intestinal perfusion (SPIP)
[Quality evaluation of Schisandra chinensis at different producing areas in northeast China].
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OBJECTIVE
To investigate the quality of Schisandra chinensis, and thus provide the useful reference data for its quality standard establishment and standardized cultivation.
METHODS
Base on the Chinese Pharmacopoeia and literature relating to S. chinensis, the appearance, water-soluble extractive,
Simultaneous determination of nine lignans from Schisandra chinensis extract using ultra-performance liquid chromatography with tandem mass spectrometry in rat plasma, urine, and gastrointestinal tract samples: application to the pharmacokinetic study of Schisandra chinensis.
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The fruit of Schisandra chinensis is a well-known herbal medicine and dietary supplement due to a variety of biological activities including antihepatotoxic and antihyperlipidemic activities. However, the simultaneous validation methodology and pharmacokinetic investigation of nine lignans of S.
[Quality evaluation and antioxidant activity research of Schisandra chinensis from various habitats].
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OBJECTIVE
To determine the contents of lignans, crude polysaccharides (CP) and total phenolic compounds (TPC) of Schisandra chinensis from various habitats in Liaoning province and evaluate their quality and free radical scavenging (FRS) activity.
METHODS
Contents of schisandrol, deoxyschizandrin
Lignans from the fruits of Schisandra chinensis (Turcz.) Baill inhibit proprotein convertase subtilisin/kexin type 9 expression.
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Bioactivity-guided fractionation of the fruits of Schisandra chinensis, using the proprotein convertase subtilisin-kexin type 9 (PCSK9) mRNA expression screening assay, led to isolation of two previously unknown lignans, 14-tigloylschinlignan D and rel-(7R, 8R, 7'R, 8'R)-manglisin E, along with 28
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