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Epidemiology of spotted wilt disease of peanut caused by Tomato spotted wilt virus in the southeastern U.S.
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Spotted wilt disease of peanut (Arachis hypogaea) (SWP), caused by Tomato spotted wilt virus (TSWV) (genus Tospovirus, family Bunyaviridae), was first observed in Alabama, Florida, and Georgia in the late 1980s and rapidly became a major limiting factor for peanut production in the region. Tobacco
Inoculation of Tomato Spotted Wilt Virus into Cotton.
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Tomato spotted wilt virus (TSWV) is an economically important virus of many crops throughout the world. Cotton, Gossypium hirsutum L., has previously been demonstrated to be susceptible to TSWV (1). During the fall of 1996, cotton was assayed as a potential host of TSWV, as it is an important early
Viral RNA synthesis in tomato spotted wilt virus-infected Nicotiana rustica plants.
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The synthesis of viral RNA species in tomato spotted wilt virus-infected Nicotiana rustica plants was followed in terms of time and relative abundance. Systemic symptoms were visible after 4 days postinoculation (p.i.), but viral (v) and viral-complementary (vc) strands of all three genomic RNA
First Report of Tomato spotted wilt virus on Tobacco in Campania, Italy.
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During the spring and summer of 2010, a survey for viruses was conducted in two tobacco field trials at the Agricultural Research and Experimentation Council in Scafati, Campania, Italy. A total of 1,392 symptomatic and asymptomatic tobacco plants (cv. Burley) were sampled, and leaves were analyzed
Toxicity of tobacco smoke to the spotted alfalfa aphid Therioaphis maculata (Buckton).
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[Some biologic characteristics of two tomato spotted wilt virus isolates].
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Biological properties of two tomato spotted wilt virus (TSWV) isolates from the tomatoes, grown on plantations of the Crimean tobacco-plant experimental stations have been investigated. These isolates were slightly differentiated by thermal inactivation point, by terms of virus storage in juice and
A protoplast system for studying tomato spotted wilt virus infection.
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A plant protoplast system for studying tomato spotted wilt tospovirus (TSWV) infection was established and tested. Using polyethylene glycol-mediated inoculation with highly infectious TSWV particles, generally 50% or more of Nicotiana rustica protoplasts were infected. In these cells viral RNA and
Pittosporum tobira: A New Host for Tomato spotted wilt virus.
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In July 1998, Pittosporum tobira shrubs, grown in a nursery in the Sharon Valley of Israel, developed foliar ring spots, mild mosaic, and tip necrosis. Of 15 samples tested for the presence of Tomato spotted wilt virus (TSWV) with a commercially available enzyme-linked immunosorbent assay (ELISA)
[Pathogenesis and induced virus resistance in tobacco plants affected by tomato spotted wilt virus].
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Pathogenecity and virulence of tomato spotted wilt virus isolated from tobacco were studied under the field conditions of the Crimea in the tobacco plants of Immunny 580 variety and Datura stramonium. As to their virulence the obtained 26 isolates were divided into three groups: strongly virulent
Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants.
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Recently high levels of protection against tomato spotted wilt virus (TSWV), a negative-strand RNA virus infecting plants, have been obtained by transforming tobacco with viral nucleoprotein (N) gene sequences. Here we demonstrate that this protection is primarily due to the presence of N gene
Different mechanisms protect transgenic tobacco against tomato spotted wilt and impatiens necrotic spot Tospoviruses.
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We generated transgenic tobacco plants expressing the sense or antisense untranslatable N coding sequence of the lettuce isolate of tomato spotted wilt virus (TSWV-BL) as well as transgenic plants containing the promoterless N gene of the virus. Both sense and antisense untranslatable N gene RNAs
[Changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus].
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As the result of electron microscope investigation of ultra-thin sections of the tissues infected by tomato spotted wilt virus it was shown that ultrastructural changes in the cells depend on the virus virulence. The isolate with low virulence induces mostly virus-specific changes (virus particles
Resistance to tomato spotted wilt virus infection in transgenic tobacco expressing the viral nucleocapsid gene.
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A recombinant plasmid containing the entire tomato spotted with virus (TSWV) nucleocapsid gene, with the exception of nucleotide encoding three N-terminal amino acids, was isolated by screening a complementary DNA library, prepared against random primed viral RNA, using a specific monoclonal
Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates.
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In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY),
Early events of tomato spotted wilt transcription and replication in protoplasts.
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A protoplast transfection system for tomato spotted wilt tospovirus (TSWV) was established by PEG-mediated infection of tobacco protoplasts. Analysis of viral RNA synthesis revealed an asymmetric production of viral (v) and viral-complementary (vc) strands of all three genomic RNA segments and a
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